Dual-mode detection of glucose based on pistol-like DNAzyme-mediated exonuclease-assisted signal cycle

Detecting glucose accurately and sensitively from clinical samples like tears and saliva is still difficult. We have created a sensor that can detect glucose with high sensitivity and accuracy by combining the use of glucose oxidase (GOx) to catalyze glucose, a pistol-like DNAzyme (PLDz) to transfor...

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Veröffentlicht in:BioTechniques. - 1988. - 76(2024), 9 vom: 05., Seite 415-423
1. Verfasser: Lu, Huiying (VerfasserIn)
Weitere Verfasser: Wang, Xiaofeng
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2024
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article AuNPs exonuclease-III glucose glucose oxidase pistol-like DNAzyme (PLDz) DNA, Catalytic Glucose IY9XDZ35W2 Gold mehr... 7440-57-5 Exodeoxyribonucleases EC 3.1.- Glucose Oxidase EC 1.1.3.4 exodeoxyribonuclease III EC 3.1.11.2
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520 |a Detecting glucose accurately and sensitively from clinical samples like tears and saliva is still difficult. We have created a sensor that can detect glucose with high sensitivity and accuracy by combining the use of glucose oxidase (GOx) to catalyze glucose, a pistol-like DNAzyme (PLDz) to transform the signal, gold nanoparticles (AuNPs) to enhance the optical properties and the exonuclease-III (Exo-III) to amplify the signal. As a result, the proposed method exhibits a low detection limit of 7.5 pM and a wide detection range covering seven orders of magnitude. The suggested dual-mode strategy provides a sensitive, precise and specific detection method for glucose. Another advantage is that the dual-mode technique significantly improves the precision and consistency of the measurements, demonstrating its immense potential for use in biomedical research and clinical diagnostics 
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