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|a 10.1080/07366205.2024.2381403
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|a eng
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1 |
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|a Lu, Huiying
|e verfasserin
|4 aut
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|a Dual-mode detection of glucose based on pistol-like DNAzyme-mediated exonuclease-assisted signal cycle
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|c 2024
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|a Text
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|a Date Completed 24.10.2024
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|a Citation Status MEDLINE
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|a Detecting glucose accurately and sensitively from clinical samples like tears and saliva is still difficult. We have created a sensor that can detect glucose with high sensitivity and accuracy by combining the use of glucose oxidase (GOx) to catalyze glucose, a pistol-like DNAzyme (PLDz) to transform the signal, gold nanoparticles (AuNPs) to enhance the optical properties and the exonuclease-III (Exo-III) to amplify the signal. As a result, the proposed method exhibits a low detection limit of 7.5 pM and a wide detection range covering seven orders of magnitude. The suggested dual-mode strategy provides a sensitive, precise and specific detection method for glucose. Another advantage is that the dual-mode technique significantly improves the precision and consistency of the measurements, demonstrating its immense potential for use in biomedical research and clinical diagnostics
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|a Journal Article
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|a AuNPs
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|a exonuclease-III
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|a glucose
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|a Exodeoxyribonucleases
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|a EC 3.1.-
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|a Glucose Oxidase
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|a exodeoxyribonuclease III
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700 |
1 |
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|a Wang, Xiaofeng
|e verfasserin
|4 aut
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773 |
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|i Enthalten in
|t BioTechniques
|d 1988
|g 76(2024), 9 vom: 05., Seite 415-423
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|x 1940-9818
|7 nnns
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|g volume:76
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|g number:9
|g day:05
|g pages:415-423
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|u http://dx.doi.org/10.1080/07366205.2024.2381403
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