Dissecting the molecular basis of the ultra-large grain formation in rice

© 2024 The Author(s). New Phytologist © 2024 New Phytologist Foundation.

Détails bibliographiques
Publié dans:The New phytologist. - 1979. - 243(2024), 6 vom: 07. Sept., Seite 2251-2264
Auteur principal: Li, Fengcheng (Auteur)
Autres auteurs: Wu, Lian, Li, Xiang, Chai, Yanan, Ruan, Nan, Wang, Ye, Xu, Na, Yu, Zhiwen, Wang, Xiaoche, Chen, Hao, Lu, Jiahao, Xu, Hai, Xu, Zhengjin, Chen, Wenfu, Xu, Quan
Format: Article en ligne
Langue:English
Publié: 2024
Accès à la collection:The New phytologist
Sujets:Journal Article de novo assembly gene pyramiding grain shape rice variety improvement Plant Proteins
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520 |a The shape of rice grains not only determines the thousand-grain weight but also correlates closely with the grain quality. Here we identified an ultra-large grain accession (ULG) with a thousand-grain weight exceeding 60 g. The integrated analysis of QTL, BSA, de novo genome assembled, transcription sequencing, and gene editing was conducted to dissect the molecular basis of the ULG formation. The ULG pyramided advantageous alleles from at least four known grain-shaping genes, OsLG3, OsMADS1, GS3, GL3.1, and one novel locus, qULG2-b, which encoded a leucine-rich repeat receptor-like kinase. The collective impacts of OsLG3, OsMADS1, GS3, and GL3.1 on grain size were confirmed in transgenic plants and near-isogenic lines. The transcriptome analysis identified 112 genes cooperatively regulated by these four genes that were prominently involved in photosynthesis and carbon metabolism. By leveraging the pleiotropy of these genes, we enhanced the grain yield, appearance, and stress tolerance of rice var. SN265. Beyond showcasing the pyramiding of multiple grain size regulation genes that can produce ULG, our study provides a theoretical framework and valuable genomic resources for improving rice variety by leveraging the pleiotropy of grain size regulated genes 
650 4 |a Journal Article 
650 4 |a de novo assembly 
650 4 |a gene pyramiding 
650 4 |a grain shape 
650 4 |a rice 
650 4 |a variety improvement 
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700 1 |a Wu, Lian  |e verfasserin  |4 aut 
700 1 |a Li, Xiang  |e verfasserin  |4 aut 
700 1 |a Chai, Yanan  |e verfasserin  |4 aut 
700 1 |a Ruan, Nan  |e verfasserin  |4 aut 
700 1 |a Wang, Ye  |e verfasserin  |4 aut 
700 1 |a Xu, Na  |e verfasserin  |4 aut 
700 1 |a Yu, Zhiwen  |e verfasserin  |4 aut 
700 1 |a Wang, Xiaoche  |e verfasserin  |4 aut 
700 1 |a Chen, Hao  |e verfasserin  |4 aut 
700 1 |a Lu, Jiahao  |e verfasserin  |4 aut 
700 1 |a Xu, Hai  |e verfasserin  |4 aut 
700 1 |a Xu, Zhengjin  |e verfasserin  |4 aut 
700 1 |a Chen, Wenfu  |e verfasserin  |4 aut 
700 1 |a Xu, Quan  |e verfasserin  |4 aut 
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