|
|
|
|
| LEADER |
01000caa a22002652c 4500 |
| 001 |
NLM375565116 |
| 003 |
DE-627 |
| 005 |
20250306112708.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
240729s2024 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1016/j.plantsci.2024.112202
|2 doi
|
| 028 |
5 |
2 |
|a pubmed25n1251.xml
|
| 035 |
|
|
|a (DE-627)NLM375565116
|
| 035 |
|
|
|a (NLM)39069009
|
| 035 |
|
|
|a (PII)S0168-9452(24)00229-2
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Jin, Feng
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Amino acid permease OsAAP12 negatively regulates rice tillers and grain yield by transporting specific amino acids to affect nitrogen and cytokinin pathways
|
| 264 |
|
1 |
|c 2024
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 15.08.2024
|
| 500 |
|
|
|a Date Revised 15.08.2024
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a Copyright © 2024 Elsevier B.V. All rights reserved.
|
| 520 |
|
|
|a Amino acids are necessary nutrients for the growth of Oryza sativa (rice), which can be mediated by amino acid transporter; however, our understanding of these transporters is still limited. This study found that the expression levels of amino acid permease gene OsAAP12 differed between indica and japonica rice. Altered expression of OsAAP12 negatively regulated tillering and yield in transgenic rice lines. Subcellular localization revealed that OsAAP12 was primarily localized to the plasma membrane. Moreover, it was indicated that OsAAP12 transported polar neutral amino acids asparagine (Asn), threonine (Thr), and serine (Ser) through experiments involving yeast heterologous complementation, fluorescence amino acid uptake, and amino acid content determination. Additionally, exogenous application of amino acids Asn, Thr, and Ser suppressed axillary buds outgrowth in OsAAP12 overexpression lines compared with wild-type ZH11. Conversely, the opposite trend was observed in CRISPR mutant lines. RNA-seq analysis showed that the expression patterns of genes involved in the nitrogen and cytokinin pathways were generally altered in OsAAP12 modified lines. Hormone assays indicated that OsAAP12 mutant lines accumulated cytokinins in the basal part of rice, whereas overexpression lines had the opposite effect. In summary, CRISPR mutant of OsAAP12 boosted rice tillering and grain yield by coordinating the content of amino acids and cytokinins, which has potential application value in high-yield rice breeding
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Amino acid
|
| 650 |
|
4 |
|a Architecture
|
| 650 |
|
4 |
|a Rice
|
| 650 |
|
4 |
|a Transporter
|
| 650 |
|
4 |
|a Yield
|
| 650 |
|
7 |
|a Cytokinins
|2 NLM
|
| 650 |
|
7 |
|a Amino Acids
|2 NLM
|
| 650 |
|
7 |
|a Nitrogen
|2 NLM
|
| 650 |
|
7 |
|a N762921K75
|2 NLM
|
| 650 |
|
7 |
|a Plant Proteins
|2 NLM
|
| 650 |
|
7 |
|a Amino Acid Transport Systems
|2 NLM
|
| 700 |
1 |
|
|a Huang, Weiting
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Xie, Pengfei
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Wu, Bowen
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Zhao, Quanzhi
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Fang, Zhongming
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Plant science : an international journal of experimental plant biology
|d 1985
|g 347(2024) vom: 30. Okt., Seite 112202
|w (DE-627)NLM098174193
|x 1873-2259
|7 nnas
|
| 773 |
1 |
8 |
|g volume:347
|g year:2024
|g day:30
|g month:10
|g pages:112202
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1016/j.plantsci.2024.112202
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 347
|j 2024
|b 30
|c 10
|h 112202
|