ABA exerts a promotive effect on the early process of somatic embryogenesis in Quercus aliena Bl

Bibliographische Detailangaben
Veröffentlicht in:	Plant physiology and biochemistry : PPB. - 1991. - 214(2024) vom: 25. Aug., Seite 108969
1. Verfasser:	Yang, Xiong (VerfasserIn)
Weitere Verfasser:	Chang, Muxi, Yang, Ning, Zhang, Qian, Ge, Yaoyao, Zhou, Huirong, Li, Guolei, Yang, Qinsong
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2024
Zugriff auf das übergeordnete Werk:	Plant physiology and biochemistry : PPB
Schlagworte:	Journal Article ABA Callose Somatic embryogenesis Transcriptome Zygotic embryo Abscisic Acid 72S9A8J5GW Plant Growth Regulators Plant Proteins

Beschreibung
Zusammenfassung:	Copyright © 2024 Elsevier Masson SAS. All rights reserved. Quercus aliena, a native Chinese tree species, is significant in industry and landscaping. However, it is traditionally propagated by seeds with many limitations, such as pest infestations, seed yield and quality. Thus, this study firstly introduces a somatic embryogenesis (SE) system for Q. aliena, enhancing its cultivation prospects. Thereinto, the development stage of zygotic embryo had a significant effect on SE, only immature embryos in 10-11 weeks after full bloom (WAF), rich in endogenous abscisic acid (ABA), could induce SE. Exogenous application ABA had positive roles in the early development process of both primary and secondary SE, while its antagonist had opposite roles. Transcriptome analysis showed that transcription regulation occupied the major position. Mfuzz cluster and WGCNA co-expression analysis showed that 24 candidate genes were involved in the SE process. The expression of the 24 genes were also affected by exogenous ABA signals, among which QaLEC2, QaCALS11 and QaSSRP1 occupied the important roles. Additionally, the callose content were also affected by exogenous ABA signals, which had significantly positive correlations with the expression of QaLEC2 and QaCALS11. This study not only established an efficient reproduction system for Q. aliena, but also revealed the difference in embryogenic ability of zygotic embryos from the aspects of transcriptome and endogenous hormone content, and lay a foundation for clarifying the molecular mechanism of SE, and provided a reference for exploring the vital roles of ABA in SE
Beschreibung:	Date Completed 04.08.2024 Date Revised 04.08.2024 published: Print-Electronic Citation Status MEDLINE
ISSN:	1873-2690
DOI:	10.1016/j.plaphy.2024.108969