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|a 10.1093/jxb/erae326
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|a eng
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|a Ahmad, Bilal
|e verfasserin
|4 aut
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|a Nuclear localization of Arabidopsis HD-Zip IV transcription factor GLABRA2 is driven by importin α
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|c 2024
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|a Date Completed 30.10.2024
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|a Date Revised 27.11.2024
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|a published: Print
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|a UpdateOf: bioRxiv. 2023 Nov 05:2023.11.03.565550. doi: 10.1101/2023.11.03.565550. - PMID 37961624
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|a Citation Status MEDLINE
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|a © The Author(s) 2024. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For commercial re-use, please contact reprintsoup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.
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|a GLABRA2 (GL2), a class IV homeodomain leucine-zipper (HD-Zip IV) transcription factor from Arabidopsis, is a developmental regulator of specialized cell types in the epidermis. GL2 contains a monopartite nuclear localization sequence (NLS) that is conserved in most HD-Zip IV members across the plants. We demonstrate that NLS mutations affect nuclear transport and result in a loss-of-function phenotypes. NLS fusions to enhanced yellow fluorescent protein (EYFP) show that it is sufficient for nuclear localization in roots and trichomes. Despite partial overlap of the NLS with the homeodomain, genetic dissection indicates that nuclear localization and DNA binding are separable functions. Affinity purification of GL2 from plants followed by MS-based proteomics identified importin α (IMPα) isoforms as potential GL2 interactors. NLS structural prediction and molecular docking studies with IMPα-3 revealed major interacting residues. Cytosolic yeast two-hybrid assays and co-immunoprecipitation experiments with recombinant proteins verified NLS-dependent interactions between GL2 and several IMPα isoforms. IMPα triple mutants (impα-1,2,3) exhibit abnormal trichome formation and defects in GL2 nuclear localization in trichomes, consistent with tissue-specific and redundant functions of IMPα isoforms. Taken together, our findings provide mechanistic evidence for IMPα-dependent nuclear localization of GL2 in Arabidopsis, a process that is critical for cell type differentiation of the epidermis
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|a Journal Article
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|a Epidermis
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|a HD-Zip IV
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|a NLS
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|a importin α
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|a root
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|a transcription factor
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|a trichome
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|a Arabidopsis Proteins
|2 NLM
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|a alpha Karyopherins
|2 NLM
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|a GL2 protein, Arabidopsis
|2 NLM
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|a Homeodomain Proteins
|2 NLM
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| 650 |
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|a Transcription Factors
|2 NLM
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|a Nuclear Localization Signals
|2 NLM
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|a Lerma-Reyes, Ruben
|e verfasserin
|4 aut
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|a Mukherjee, Thiya
|e verfasserin
|4 aut
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|a Nguyen, Hieu V
|e verfasserin
|4 aut
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|a Weber, Audra L
|e verfasserin
|4 aut
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|a Cummings, Emily E
|e verfasserin
|4 aut
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|a Schulze, Waltraud X
|e verfasserin
|4 aut
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|a Comer, Jeffrey R
|e verfasserin
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|a Schrick, Kathrin
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 75(2024), 20 vom: 30. Okt., Seite 6441-6461
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|x 1460-2431
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|g volume:75
|g year:2024
|g number:20
|g day:30
|g month:10
|g pages:6441-6461
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|u http://dx.doi.org/10.1093/jxb/erae326
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