Mechanical forces orchestrate the metabolism of the developing oilseed rape embryo

Mechanical forces orchestrate the metabolism of the developing oilseed rape embryo

© 2024 The Author(s). New Phytologist © 2024 New Phytologist Foundation.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 244(2024), 4 vom: 21. Okt., Seite 1328-1344
1. Verfasser: Rolletschek, Hardy (VerfasserIn)
Weitere Verfasser: Muszynska, Aleksandra, Schwender, Jörg, Radchuk, Volodymyr, Heinemann, Björn, Hilo, Alexander, Plutenko, Iaroslav, Keil, Peter, Ortleb, Stefan, Wagner, Steffen, Kalms, Laura, Gündel, André, Shi, Hai, Fuchs, Jörg, Szymanski, Jedrzej Jakub, Braun, Hans-Peter, Borisjuk, Ljudmilla
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2024
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Brassica napus MRI computer graphic reconstruction embryogenesis maturation mechanical stress morphogenesis transcription factors Plant Proteins
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520 |a The initial free expansion of the embryo within a seed is at some point inhibited by its contact with the testa, resulting in its formation of folds and borders. Although less obvious, mechanical forces appear to trigger and accelerate seed maturation. However, the mechanistic basis for this effect remains unclear. Manipulation of the mechanical constraints affecting either the in vivo or in vitro growth of oilseed rape embryos was combined with analytical approaches, including magnetic resonance imaging and computer graphic reconstruction, immunolabelling, flow cytometry, transcriptomic, proteomic, lipidomic and metabolomic profiling. Our data implied that, in vivo, the imposition of mechanical restraints impeded the expansion of testa and endosperm, resulting in the embryo's deformation. An acceleration in embryonic development was implied by the cessation of cell proliferation and the stimulation of lipid and protein storage, characteristic of embryo maturation. The underlying molecular signature included elements of cell cycle control, reactive oxygen species metabolism and transcriptional reprogramming, along with allosteric control of glycolytic flux. Constricting the space allowed for the expansion of in vitro grown embryos induced a similar response. The conclusion is that the imposition of mechanical constraints over the growth of the developing oilseed rape embryo provides an important trigger for its maturation 
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650 4 |a Brassica napus 
650 4 |a MRI 
650 4 |a computer graphic reconstruction 
650 4 |a embryogenesis 
650 4 |a maturation 
650 4 |a mechanical stress 
650 4 |a morphogenesis 
650 4 |a transcription factors 
650 7 |a Plant Proteins  |2 NLM 
700 1 |a Muszynska, Aleksandra  |e verfasserin  |4 aut 
700 1 |a Schwender, Jörg  |e verfasserin  |4 aut 
700 1 |a Radchuk, Volodymyr  |e verfasserin  |4 aut 
700 1 |a Heinemann, Björn  |e verfasserin  |4 aut 
700 1 |a Hilo, Alexander  |e verfasserin  |4 aut 
700 1 |a Plutenko, Iaroslav  |e verfasserin  |4 aut 
700 1 |a Keil, Peter  |e verfasserin  |4 aut 
700 1 |a Ortleb, Stefan  |e verfasserin  |4 aut 
700 1 |a Wagner, Steffen  |e verfasserin  |4 aut 
700 1 |a Kalms, Laura  |e verfasserin  |4 aut 
700 1 |a Gündel, André  |e verfasserin  |4 aut 
700 1 |a Shi, Hai  |e verfasserin  |4 aut 
700 1 |a Fuchs, Jörg  |e verfasserin  |4 aut 
700 1 |a Szymanski, Jedrzej Jakub  |e verfasserin  |4 aut 
700 1 |a Braun, Hans-Peter  |e verfasserin  |4 aut 
700 1 |a Borisjuk, Ljudmilla  |e verfasserin  |4 aut 
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