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240724s2024 xx |||||o 00| ||eng c |
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|a 10.1111/nph.19976
|2 doi
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|a pubmed25n1250.xml
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|a (DE-627)NLM375322361
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|a (NLM)39044689
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Xue, Feiyang
|e verfasserin
|4 aut
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| 245 |
1 |
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|a m6A demethylase OsALKBH5 is required for double-strand break formation and repair by affecting mRNA stability in rice meiosis
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|c 2024
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 21.11.2024
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|a Date Revised 21.11.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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| 520 |
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|a © 2024 The Author(s). New Phytologist © 2024 New Phytologist Foundation.
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|a N6-methyladenosine (m6A) RNA modification is the most prevalent messenger RNA (mRNA) modification in eukaryotes and plays critical roles in the regulation of gene expression. m6A is a reversible RNA modification that is deposited by methyltransferases (writers) and removed by demethylases (erasers). The function of m6A erasers in plants is highly diversified and their roles in cereal crops, especially in reproductive development essential for crop yield, are largely unknown. Here, we demonstrate that rice OsALKBH5 acts as an m6A demethylase required for the normal progression of male meiosis. OsALKBH5 is a nucleo-cytoplasmic protein, highly enriched in rice anthers during meiosis, that associates with P-bodies and exon junction complexes, suggesting that it is involved in regulating mRNA processing and abundance. Mutations of OsALKBH5 cause reduced double-strand break (DSB) formation, severe defects in DSB repair, and delayed meiotic progression, leading to complete male sterility. Transcriptome analysis and m6A profiling indicate that OsALKBH5-mediated m6A demethylation stabilizes the mRNA level of multiple meiotic genes directly or indirectly, including several genes that regulate DSB formation and repair. Our study reveals the indispensable role of m6A metabolism in post-transcriptional regulation of meiotic progression in rice
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|a Journal Article
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|a DSB
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4 |
|a OsALKBH5
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4 |
|a m6A
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|a meiosis
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|a rice
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| 650 |
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7 |
|a Plant Proteins
|2 NLM
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| 650 |
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|a N-methyladenosine
|2 NLM
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| 650 |
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|a CLE6G00625
|2 NLM
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| 650 |
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|a Adenosine
|2 NLM
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| 650 |
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7 |
|a K72T3FS567
|2 NLM
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| 650 |
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|a RNA, Messenger
|2 NLM
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| 700 |
1 |
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|a Zhang, Jie
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wu, Di
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Sun, Shiyu
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Fu, Ming
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Jie
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Searle, Iain
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Gao, Hongbo
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Liang, Wanqi
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t The New phytologist
|d 1979
|g 244(2024), 6 vom: 03. Dez., Seite 2326-2342
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnas
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| 773 |
1 |
8 |
|g volume:244
|g year:2024
|g number:6
|g day:03
|g month:12
|g pages:2326-2342
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| 856 |
4 |
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|u http://dx.doi.org/10.1111/nph.19976
|3 Volltext
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|d 244
|j 2024
|e 6
|b 03
|c 12
|h 2326-2342
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