The methyl jasmonate-responsive transcription factor SmERF106 promotes tanshinone accumulation in Salvia miltiorrhiza

Bibliographische Detailangaben
Veröffentlicht in:	Plant physiology and biochemistry : PPB. - 1991. - 214(2024) vom: 05. Aug., Seite 108932
1. Verfasser:	Li, Yajing (VerfasserIn)
Weitere Verfasser:	Cao, Jiajia, Zhang, Yuchen, Liu, Yiru, Gao, Shouhong, Zhang, Pan, Xia, Wenwen, Zhang, Ke, Yang, Xu, Wang, Yun, Zhang, Lei, Li, Bo, Li, Tingzhao, Xiao, Ying, Chen, Junfeng, Chen, Wansheng
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2024
Zugriff auf das übergeordnete Werk:	Plant physiology and biochemistry : PPB
Schlagworte:	Journal Article DAP-Seq ERF transcription factors Hairy root MeJA Transcriptional regulation Abietanes tanshinone 03UUH3J385 Oxylipins mehr... methyl jasmonate 900N171A0F Transcription Factors Cyclopentanes Plant Proteins Acetates

Beschreibung
Zusammenfassung:	Copyright © 2024. Published by Elsevier Masson SAS. Understanding the regulatory biosynthesis mechanisms of active compounds in herbs is vital for the preservation and sustainable use of natural medicine resources. Diterpenoids, which play a key role in plant growth and resistance, also serve as practical products for humans. Tanshinone, a class of abietane-type diterpenes unique to the Salvia genus, such as Salvia miltiorrhiza, is an excellent model for studying diterpenoids. In this study, we discovered that a transcription factor, SmERF106, responds to MeJA induction and is located in the nucleus. It exhibits a positive correlation with the expression of SmKSL1 and SmIDI1, which are associated with tanshinone biosynthesis. We performed DNA affinity purification sequencing (DAP-seq) to predict genes that may be transcriptionally regulated by SmERF106. Our cis-elements analysis suggested that SmERF106 might bind to GCC-boxes in the promoters of SmKSL1 and SmIDI1. This indicates that SmKSL1 and SmIDI1 could be potential target genes regulated by SmERF106 in the tanshinone biosynthesis pathway. Their interaction was then demonstrated through a series of in vitro and in vivo binding experiments, including Y1H, EMSA, and Dual-LUC. Overexpression of SmERF106 in the hairy root of S. miltiorrhiza led to a significant increase in tanshinone content and the transcriptional levels of SmKSL1 and SmIDI1. In summary, we found that SmERF106 can activate the transcription of SmKSL1 and SmIDI1 in response to MeJA induction, thereby promoting tanshinone biosynthesis. This discovery provides new insights into the regulatory mechanisms of tanshinones in response to JA and offers a potential gene tool for tanshinone metabolic engineering strategy
Beschreibung:	Date Completed 04.08.2024 Date Revised 05.08.2024 published: Print-Electronic Citation Status MEDLINE
ISSN:	1873-2690
DOI:	10.1016/j.plaphy.2024.108932