Adipocyte ABCA1 expression analysis using flow cytometry
Adipocyte characterization and assessing membrane proteins using flow cytometry has been proven to be challenging as adipocytes are fragile, especially in subjects with high BMI. We overcame these challenges through a protocol optimizing tissue digestion time by reducing intermediate steps to minimi...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1993. - 76(2024), 8 vom: 17., Seite 405-410
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| 1. Verfasser: |
Shukla, Sakshi
(VerfasserIn) |
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| Weitere Verfasser: |
Bansal, Ashutosh,
Aggarwal, Sandeep,
Singh, Archna |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2024
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
ABCA1
BMI
adipocytes
adipose tissue
flow cytometry
gating
lipid droplets
ATP Binding Cassette Transporter 1
Abca1 protein, mouse
ABCA1 protein, human |
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