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240717s2024 xx |||||o 00| ||eng c |
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|a 10.1111/nph.19970
|2 doi
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|a pubmed25n1249.xml
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|a DE-627
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Du, Qian
|e verfasserin
|4 aut
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| 245 |
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|a A transcriptional repressor HVA regulates vascular bundle formation through auxin transport in Arabidopsis stem
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|c 2024
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 01.08.2024
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|a Date Revised 10.09.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2024 The Author(s). New Phytologist © 2024 New Phytologist Foundation.
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|a Vascular bundles transport water and photosynthate to all organs, and increased bundle number contributes to crop lodging resistance. However, the regulation of vascular bundle formation is poorly understood in the Arabidopsis stem. We report a novel semi-dominant mutant with high vascular activity, hva-d, showing increased vascular bundle number and enhanced cambium proliferation in the stem. The activation of a C2H2 zinc finger transcription factor, AT5G27880/HVA, is responsible for the hva-d phenotype. Genetic, biochemical, and fluorescent microscopic analyses were used to dissect the functions of HVA. HVA functions as a repressor and interacts with TOPLESS via the conserved Ethylene-responsive element binding factor-associated Amphiphilic Repression motif. In contrast to the HVA activation line, knockout of HVA function with a CRISPR-Cas9 approach or expression of HVA fused with an activation domain VP16 (HVA-VP16) resulted in fewer vascular bundles. Further, HVA directly regulates the expression of the auxin transport efflux facilitator PIN1, as a result affecting auxin accumulation. Genetics analysis demonstrated that PIN1 is epistatic to HVA in controlling bundle number. This research identifies HVA as a positive regulator of vascular initiation through negatively modulating auxin transport and sheds new light on the mechanism of bundle formation in the stem
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|a Journal Article
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|a Arabidopsis
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|a cambium activity
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|a regulation
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|a transcription factor
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|a vascular development
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| 650 |
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|a Arabidopsis Proteins
|2 NLM
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| 650 |
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|a Indoleacetic Acids
|2 NLM
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| 650 |
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|a Repressor Proteins
|2 NLM
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| 650 |
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|a PIN1 protein, Arabidopsis
|2 NLM
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| 650 |
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|a Membrane Transport Proteins
|2 NLM
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| 650 |
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|a Transcription Factors
|2 NLM
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| 700 |
1 |
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|a Yuan, Bingjian
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Thapa Chhetri, Gaurav
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Tong
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Qi, Liying
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Huanzhong
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t The New phytologist
|d 1979
|g 243(2024), 5 vom: 16. Sept., Seite 1681-1697
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnas
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| 773 |
1 |
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|g volume:243
|g year:2024
|g number:5
|g day:16
|g month:09
|g pages:1681-1697
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| 856 |
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|u http://dx.doi.org/10.1111/nph.19970
|3 Volltext
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