ZmSPL10, ZmSPL14 and ZmSPL26 act together to promote stigmatic papilla formation in maize through regulating auxin signaling and ZmWOX3A expression

Bibliographische Detailangaben
Veröffentlicht in:	The New phytologist. - 1979. - 243(2024), 5 vom: 02. Sept., Seite 1870-1886
1. Verfasser:	Kong, Dexin (VerfasserIn)
Weitere Verfasser:	Jing, Yifeng, Duan, Yaping, He, Mengqi, Ding, Hui, Li, Heying, Zhong, Zhuojun, Zheng, Zhigang, Fan, Xiuying, Pan, Xuan, Li, Yanqun, Bai, Mei, Li, Xinjian, Luo, Minhua, Xue, Weicong, Zhang, Xiaoming, Xu, Xin, Yuan, Yateng, Zou, Ting, Chen, Lihong, Ding, Wenyan, Zhao, Yongping, Wang, Baobao, Wu, Hong, Liu, Qing, Wang, Haiyang
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2024
Zugriff auf das übergeordnete Werk:	The New phytologist
Schlagworte:	Journal Article ZmSPL10 ZmSPL14 ZmSPL26 ZmWOX3A Maize (Zea mays L.) auxin stigmatic papilla Indoleacetic Acids Plant Proteins

Beschreibung
Zusammenfassung:	© 2024 The Author(s). New Phytologist © 2024 New Phytologist Foundation. Maize silk is a specialized type of stigma, covered with numerous papillae for pollen grain capture. However, the developmental process of stigmatic papillae and the underlying regulatory mechanisms have remained largely unknown. Here, we combined the cytological, genetic and molecular studies to demonstrate that three homologous genes ZmSPL10, ZmSPL14 and ZmSPL26 play a central role in promoting stigmatic papilla formation in maize. We show that their triple knockout mutants are nearly complete lack of stigmatic papilla, resulting in a severe reduction in kernel setting. Cellular examination reveals that stigmatic papilla is developed from a precursor cell, which is the smaller daughter cell resulting from asymmetric cell division of a silk epidermal cell. In situ hybridization shows that ZmSPL10, ZmSPL14 and their target genes SPI1, ZmPIN1b, ZmARF28 and ZmWOX3A are preferentially expressed in the precursor cells of stigmatic papillae. Moreover, ZmSPL10, ZmSPL14 and ZmSPL26 directly bind to the promoters of SPI1, ZmPIN1b, ZmARF28 and ZmWOX3A and promote their expression. Further, Zmwox3a knockout mutants display severe defects in stigmatic papilla formation and reduced seed setting. Collectively, our results demonstrate that ZmSPL10, ZmSPL14 and ZmSPL26 act together to promote stigmatic papilla development through regulating auxin signaling and ZmWOX3A expression
Beschreibung:	Date Completed 01.08.2024 Date Revised 10.09.2024 published: Print-Electronic Citation Status MEDLINE
ISSN:	1469-8137
DOI:	10.1111/nph.19961