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240715s2024 xx |||||o 00| ||eng c |
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|a 10.2166/wst.2024.203
|2 doi
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|a pubmed24n1471.xml
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|a (DE-627)NLM374950466
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|a (NLM)39007315
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Subroyen, Sueyanka
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Evaluating storage conditions and enhancement strategies on viral biomarker recovery for WBE applications
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|c 2024
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 15.07.2024
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|a Date Revised 15.07.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2024 The Authors This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC BY 4.0), which permits copying, adaptation and redistribution, provided the original work is properly cited (http://creativecommons.org/licenses/by/4.0/).
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|a Wastewater-based epidemiology (WBE) is a valuable disease surveillance tool. However, little is known on how factors such as transportation, storage, and wastewater characteristics influence the accuracy of the quantification methods. Hence, this study investigated the impact of storage temperatures and physicochemical characteristics of wastewater on SARS-CoV-2 and influenza A stability using droplet digital PCR. Additionally, strategies to enhance viral recovery were explored. Municipal influent wastewater stored between ±25 and -80 °C was assessed for a period of 84 days to determine viral degradation. Degradation up to 94.1% of influenza A and SARS-CoV-2 was observed in all samples with the highest at ±25 °C. Viral degradation was correlated to the changes in wastewater physicochemical characteristics. The low degradation observed of SARS-CoV-2 in the spiked pellets were indicative of viral adhesion to wastewater solids, which correlated with changes in pH. Ultrasonication frequencies ranging from 4 to 16 kHz, increased SARS-CoV-2 concentrations in the supernatant between 3.30 and 35.65%, indicating viral RNA attachment to wastewater solids. These results highlight the importance of additional pretreatment methods for maximizing RNA recovery from wastewater samples. Based on these findings, it was deduced that wastewater preservation studies are essential, and pretreatment should be included in the WBE methodology
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|a Journal Article
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|a COVID-19
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|a SARS-CoV-2
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4 |
|a droplet digital PCR
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| 650 |
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4 |
|a influenza A
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| 650 |
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4 |
|a wastewater-based epidemiology
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| 650 |
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7 |
|a Wastewater
|2 NLM
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| 650 |
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|a RNA, Viral
|2 NLM
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| 650 |
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7 |
|a Biomarkers
|2 NLM
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| 700 |
1 |
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|a Pillay, Leanne
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Bux, Faizal
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kumari, Sheena
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Water science and technology : a journal of the International Association on Water Pollution Research
|d 1986
|g 90(2024), 1 vom: 15. Juli, Seite 213-224
|w (DE-627)NLM098149431
|x 0273-1223
|7 nnns
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| 773 |
1 |
8 |
|g volume:90
|g year:2024
|g number:1
|g day:15
|g month:07
|g pages:213-224
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|u http://dx.doi.org/10.2166/wst.2024.203
|3 Volltext
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