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240714s2024 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2024.108929
|2 doi
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|a pubmed24n1492.xml
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|a (DE-627)NLM374900280
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|e rakwb
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|a eng
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|a Qin, Shuangshuang
|e verfasserin
|4 aut
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|a Analysis of the Spatholobus suberectus full-length transcriptome identified an R2R3-MYB transcription factor-encoding gene SsMYB158 that regulates flavonoid biosynthesis
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|c 2024
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|a Text
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 04.08.2024
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|a Date Revised 04.08.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2024 Elsevier Masson SAS. All rights reserved.
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|a Spatholobus suberectus Dunn (Leguminosae) has been used for medicinal purposes for a long period. Flavonoids are the major bioactive components of S. suberectus. However, there is still limited knowledge of the exact method via which transcription factors (TFs) regulate flavonoid biosynthesis. The full-length transcriptome of S. suberectus was analyzed using SMRT sequencing; 61,548 transcripts were identified, including 12,311 new gene loci, 53,336 novel transcripts, 44,636 simple sequence repeats, 36,414 complete coding sequences, 871 long non-coding RNAs and 6781 TFs. The SsMYB158 TF, which is associated with flavonoid biosynthesis, belongs to the R2R3-MYB class and is localized subcellularly to the nucleus. The overexpression of SsMYB158 in Nicotiana benthamiana and the transient overexpression of SsMYB158 in S. suberectus resulted in a substantial enhancement in both flavonoids and catechin levels. In addition, there was a remarkable upregulation in the expression of essential enzyme-coding genes associated with the flavonoid biosynthesis pathways. Our study revealed SsMYB158 as a critical regulator of flavonoid biosynthesis in S. suberectus and laying the foundation for its molecular breeding
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|a Journal Article
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|a Flavonoid biosynthesis
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|a Full-length transcriptome
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|a Spatholobus suberectus dunn
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|a SsMYB158
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|a Transcription factor
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|a Flavonoids
|2 NLM
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|a Transcription Factors
|2 NLM
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|a Plant Proteins
|2 NLM
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|a Wei, Guili
|e verfasserin
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|a Lin, Quan
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|a Tang, Danfeng
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|a Li, Cui
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|a Tan, Zhien
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|a Yao, Lixiang
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|a Huang, Lirong
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|4 aut
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|a Wei, Fan
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|a Liang, Ying
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 214(2024) vom: 23. Aug., Seite 108929
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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|g volume:214
|g year:2024
|g day:23
|g month:08
|g pages:108929
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|u http://dx.doi.org/10.1016/j.plaphy.2024.108929
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