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240712s2024 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2024.108924
|2 doi
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|a pubmed24n1492.xml
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|a (DE-627)NLM374793220
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|a (NLM)38991593
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|a (PII)S0981-9428(24)00592-8
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Guo, Ying
|e verfasserin
|4 aut
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|a The transcription factor PagLBD4 represses cell differentiation and secondary cell wall biosynthesis in Populus
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|c 2024
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 04.08.2024
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|a Date Revised 04.08.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2024 Elsevier Masson SAS. All rights reserved.
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|a LBD (LATERAL ORGAN BOUNDARIES DOMAIN) transcription factors are key regulators of plant growth and development. In this study, we functionally characterized the PagLBD4 gene in Populus (Populus alba × Populus glandulosa). Overexpression of PagLBD4 (PagLBD4OE) significantly repressed secondary xylem differentiation and secondary cell wall (SCW) deposition, while CRISPR/Cas9-mediated PagLBD4 knockout (PagLBD4KO) significantly increased secondary xylem differentiation and SCW deposition. Consistent with the functional analysis, gene expression analysis revealed that SCW biosynthesis pathways were significantly down-regulated in PagLBD4OE plants but up-regulated in PagLBD4KO plants. We also performed DNA affinity purification followed by sequencing (DAP-seq) to identify genes bound by PagLBD4. Integration of RNA sequencing (RNA-seq) and DAP-seq data identified 263 putative direct target genes (DTGs) of PagLBD4, including important regulatory genes for SCW biosynthesis, such as PagMYB103 and PagIRX12. Together, our results demonstrated that PagLBD4 is a repressor of secondary xylem differentiation and SCW biosynthesis in Populus, which possibly lead to the dramatic growth repression in PagLBD4OE plants
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|a Journal Article
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|a DAP-Seq
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|a LBD domain transcription factor
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|a Populus
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|a RNA-Seq
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|a Secondary cell wall
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|a Plant Proteins
|2 NLM
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|a Transcription Factors
|2 NLM
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| 700 |
1 |
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|a Yao, Lijuan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chen, Xiaoman
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Xu, Xiaoqi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Sang, Ya Lin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Liu, Li-Jun
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 214(2024) vom: 09. Aug., Seite 108924
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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|g volume:214
|g year:2024
|g day:09
|g month:08
|g pages:108924
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|u http://dx.doi.org/10.1016/j.plaphy.2024.108924
|3 Volltext
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