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240711s2024 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2024.108913
|2 doi
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|a pubmed24n1492.xml
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|a (DE-627)NLM374739722
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|a (NLM)38986239
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|a (PII)S0981-9428(24)00581-3
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Liu, Zhi
|e verfasserin
|4 aut
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| 245 |
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|a PuERF008-PuFAD2 module regulates aroma formation via the fatty acid pathway in response to calcium signaling in 'Nanguo' pear
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|c 2024
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 04.08.2024
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|a Date Revised 04.08.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2024 Elsevier Masson SAS. All rights reserved.
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|a Calcium acts as a secondary messenger in plants and is essential for plant growth and development. However, studies on the pathway of aroma synthesis in 'Nanguo' pear (Pyrus ussriensis Maxim.) are scarce. In this study, a bioinformatics analysis of transcriptomic data from calcium-treated 'Nanguo' pear was performed, which identified two fatty acid desaturases, PuFAD2 and PuFAD3, and eight AP2/ERF transcription factors, all exhibiting the same expression patterns. Transient expression experiments showed overexpression of PuFAD2 and PuFAD3 significantly increased the levels of aromatic substrates linoleic acid, hexanal, linolenic acid, and (E)-2-hexenal, but RNAi (RNA interference) had the opposite expression. Promoter sequences analysis revealed that PuFAD2 and PuFAD3 have ERE (estrogen response element) motifs on their promoters. The strongest activation of PuFAD2 by PuERF008 was verified using a dual-luciferase reporting system. Additionally, yeast one-hybrid and electrophoretic mobility shift assays revealed PuERF008 could active PuFAD2. Transient overexpression and RNAi analyses of PuERF008 showed a strong correlation with the expression of PuFAD2. This study provides insights into the process of aroma biosynthesis in 'Nanguo' pear and offers a theoretical basis for elucidating the role of calcium signaling in aroma synthesis
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|a Journal Article
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|a Aroma
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|a Calcium signaling
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|a PuERF008-PuFAD2
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|a Unsaturated fatty acid metabolic pathway
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|a ‘Nanguo’ pear
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|a Plant Proteins
|2 NLM
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|a Fatty Acids
|2 NLM
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|a Transcription Factors
|2 NLM
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|a Fatty Acid Desaturases
|2 NLM
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|a EC 1.14.19.-
|2 NLM
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| 650 |
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|a Calcium
|2 NLM
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| 650 |
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|a SY7Q814VUP
|2 NLM
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| 700 |
1 |
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|a Qin, Gai-Hua
|e verfasserin
|4 aut
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1 |
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|a Wei, Shu-Wei
|e verfasserin
|4 aut
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1 |
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|a Yan, Long-Fei
|e verfasserin
|4 aut
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1 |
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|a Ning, Zhi-Yuan
|e verfasserin
|4 aut
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|a Wang, Hong
|e verfasserin
|4 aut
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1 |
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|a Cao, Zhen
|e verfasserin
|4 aut
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|a Hu, Kang-Di
|e verfasserin
|4 aut
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|a Yao, Gai-Fang
|e verfasserin
|4 aut
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| 700 |
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|a Zhang, Hua
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 214(2024) vom: 01. Aug., Seite 108913
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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| 773 |
1 |
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|g volume:214
|g year:2024
|g day:01
|g month:08
|g pages:108913
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|u http://dx.doi.org/10.1016/j.plaphy.2024.108913
|3 Volltext
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