Rare variations within the serine/arginine-rich splicing factor PtoRSZ21 modulate stomatal size to determine drought tolerance in Populus

Rare variations within the serine/arginine-rich splicing factor PtoRSZ21 modulate stomatal size to determine drought tolerance in Populus

© 2024 The Author(s). New Phytologist © 2024 New Phytologist Foundation.

Détails bibliographiques
Publié dans:The New phytologist. - 1979. - 243(2024), 5 vom: 01. Sept., Seite 1776-1794
Auteur principal: Huang, Rui (Auteur)
Autres auteurs: Jin, Zhuoying, Zhang, Donghai, Li, Lianzheng, Zhou, Jiaxuan, Xiao, Liang, Li, Peng, Zhang, Mengjiao, Tian, Chongde, Zhang, Wenke, Zhong, Leishi, Quan, Mingyang, Zhao, Rui, Du, Liang, Liu, Li-Jun, Li, Zhonghai, Zhang, Deqiang, Du, Qingzhang
Format: Article en ligne
Langue:English
Publié: 2024
Accès à la collection:The New phytologist
Sujets:Journal Article Populus alternative splicing drought tolerance missing heritability rare variants stomatal size Plant Proteins Serine-Arginine Splicing Factors 170974-22-8
Description
Résumé:© 2024 The Author(s). New Phytologist © 2024 New Phytologist Foundation.
Rare variants contribute significantly to the 'missing heritability' of quantitative traits. The genome-wide characteristics of rare variants and their roles in environmental adaptation of woody plants remain unexplored. Utilizing genome-wide rare variant association study (RVAS), expression quantitative trait loci (eQTL) mapping, genetic transformation, and molecular experiments, we explored the impact of rare variants on stomatal morphology and drought adaptation in Populus. Through comparative analysis of five world-wide Populus species, we observed the influence of mutational bias and adaptive selection on the distribution of rare variants. RVAS identified 75 candidate genes correlated with stomatal size (SS)/stomatal density (SD), and a rare haplotype in the promoter of serine/arginine-rich splicing factor PtoRSZ21 emerged as the foremost association signal governing SS. As a positive regulator of drought tolerance, PtoRSZ21 can recruit the core splicing factor PtoU1-70K to regulate alternative splicing (AS) of PtoATG2b (autophagy-related 2). The rare haplotype PtoRSZ21hap2 weakens binding affinity to PtoMYB61, consequently affecting PtoRSZ21 expression and SS, ultimately resulting in differential distribution of Populus accessions in arid and humid climates. This study enhances the understanding of regulatory mechanisms that underlie AS induced by rare variants and might provide targets for drought-tolerant varieties breeding in Populus
Description:Date Completed 01.08.2024
Date Revised 01.08.2024
published: Print-Electronic
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.19934