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|a 10.2478/jofnem-2024-0023
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|a eng
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|a Subbotin, Sergei A
|e verfasserin
|4 aut
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| 245 |
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|a Recombinase Polymerase Amplification assay for detection of the British root-knot nematode, Meloidogyne artiellia
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|c 2024
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|a Text
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|a Date Revised 25.11.2024
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|a published: Electronic-eCollection
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|a Citation Status PubMed-not-MEDLINE
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|a © 2024 Sergei A. Subbotin et al., published by Sciendo.
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|a Recombinase polymerase amplification (RPA) is an isothermal in vitro nucleic acid amplification technique that has been adopted for simple, robust, rapid, reliable diagnostics of nematodes. In this study, the real-time RPA assay and RPA assay combined with lateral flow dipsticks (LF-RPA) have been developed targeting the ITS rRNA gene of the British root-knot nematode, Meloidogyne artiellia. The assay provided specific and rapid detection of this root-knot nematode species from crude nematode extracts without a DNA extraction step with a sensitivity of 0.125 second-stage juvenile (J2) specimen per a reaction tube for real-time RPA during 11 min and a sensitivity of 0.5 J2 specimens per a reaction tube for LF-RPA during 25 min. The RPA assays were validated with a wide range of non-target root-knot nematodes. The LF-RPA assay has great potential for nematode diagnostics in the laboratory having minimal available equipment
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|a Journal Article
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|a ITS rRNA
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|a RPA
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|a chickpea
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|a diagnostics
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|a species-specific primer
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| 700 |
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|a Palomares-Rius, Juan E
|e verfasserin
|4 aut
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| 700 |
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|a Castillo, Pablo
|e verfasserin
|4 aut
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|i Enthalten in
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|u http://dx.doi.org/10.2478/jofnem-2024-0023
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