RNA HELICASE 32 is essential for female gametophyte development in Arabidopsis

Bibliographische Detailangaben
Veröffentlicht in:	Plant science : an international journal of experimental plant biology. - 1985. - 347(2024) vom: 28. Aug., Seite 112183
1. Verfasser:	Yuan, Jinhong (VerfasserIn)
Weitere Verfasser:	Jin, Huijie, Tian, Munan, Li, Daiyu, Meng, Yao, Zhou, Huihui, Liu, Meng, Meng, Deqing, Wei, Yunliang, Feng, Liuchun, Sang, Shifei, Chen, Changbin, Ji, Shengdong, Li, Junhua
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2024
Zugriff auf das übergeordnete Werk:	Plant science : an international journal of experimental plant biology
Schlagworte:	Journal Article Arabidopsis Cell cycle Female gametogenesis RNA helicase Vacuole biogenesis Arabidopsis Proteins RNA Helicases EC 3.6.4.13

Beschreibung
Zusammenfassung:	Copyright © 2024 Elsevier B.V. All rights reserved. The normal progression of mitotic cycles and synchronized development within female reproductive organs are pivotal for sexual reproduction in plants. Nevertheless, our understanding of the genetic regulation governing mitotic cycles during the haploid phase of higher plants remains limited. In this study, we characterized RNA HELICASE 32 (RH32), which plays an essential role in female gametogenesis in Arabidopsis. The rh32 heterozygous mutant was semi-sterile, whereas the homozygous mutant was nonviable. The rh32 mutant allele could be transmitted through the male gametophyte, but not the female gametophyte. Phenotypic analysis revealed impaired mitotic progression, synchronization, and cell specification in rh32 female gametophytes, causing the arrest of embryo sacs. In the delayed pollination test, none of the retarded embryo sacs developed into functional female gametophytes, and the vast majority of rh32 female gametophytes were defective in the formation of the large central vacuole. RH32 is strongly expressed in the embryo sac. Knock-down of RH32 resulted in the accumulation of unprocessed 18 S pre-rRNA, implying that RH32 is involved in ribosome synthesis. Based on these findings, we propose that RH32 plays a role in ribosome synthesis, which is critical for multiple processes in female gametophyte development
Beschreibung:	Date Completed 15.08.2024 Date Revised 15.08.2024 published: Print-Electronic Citation Status MEDLINE
ISSN:	1873-2259
DOI:	10.1016/j.plantsci.2024.112183