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240704s2024 xx |||||o 00| ||eng c |
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|a 10.1002/adma.202401559
|2 doi
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|a pubmed24n1502.xml
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|a (DE-627)NLM374459401
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|a (NLM)38958107
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Zhang, Shuang
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Boronic Acid-Rich Lanthanide Metal-Organic Frameworks Enable Deep Proteomics with Ultratrace Biological Samples
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|c 2024
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 15.08.2024
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|a Date Revised 15.08.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2024 Wiley‐VCH GmbH.
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|a Label-free proteomics is widely used to identify disease mechanism and potential therapeutic targets. However, deep proteomics with ultratrace clinical specimen remains a major technical challenge due to extensive contact loss during complex sample pretreatment. Here, a hybrid of four boronic acid-rich lanthanide metal-organic frameworks (MOFs) with high protein affinity is introduced to capture proteins in ultratrace samples jointly by nitrogen-boronate complexation, cation-π and ionic interactions. A MOFs Aided Sample Preparation (MASP) workflow that shrinks sample volume and integrates lysis, protein capture, protein digestion and peptide collection steps into a single PCR tube to minimize sample loss caused by non-specific absorption, is proposed further. MASP is validated to quantify ≈1800 proteins in 10 HEK-293T cells. MASP is applied to profile cerebrospinal fluid (CSF) proteome from cerebral stroke and brain damaged patients, and identified ≈3700 proteins in 1 µL CSF. MASP is further demonstrated to detect ≈9600 proteins in as few as 50 µg mouse brain tissues. MASP thus enables deep, scalable, and reproducible proteome on precious clinical samples with low abundant proteins
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|a Journal Article
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|a metal‐organic frameworks (MOFs)
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|a protein absorption
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| 650 |
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4 |
|a proteomics
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4 |
|a trace samples
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| 650 |
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|a Metal-Organic Frameworks
|2 NLM
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| 650 |
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|a Boronic Acids
|2 NLM
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| 650 |
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|a Lanthanoid Series Elements
|2 NLM
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| 650 |
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|a Proteome
|2 NLM
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| 700 |
1 |
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|a Ghalandari, Behafarid
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chen, Youming
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Qingwen
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Liu, Kun
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Sun, Xinyi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ding, Xinwen
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Song, Sunfengda
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Jiang, Lai
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ding, Xianting
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Advanced materials (Deerfield Beach, Fla.)
|d 1998
|g 36(2024), 33 vom: 01. Aug., Seite e2401559
|w (DE-627)NLM098206397
|x 1521-4095
|7 nnns
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| 773 |
1 |
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|g volume:36
|g year:2024
|g number:33
|g day:01
|g month:08
|g pages:e2401559
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| 856 |
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|u http://dx.doi.org/10.1002/adma.202401559
|3 Volltext
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