N-terminal acetylation orchestrates glycolate-mediated ROS homeostasis to promote rice thermoresponsive growth

N-terminal acetylation orchestrates glycolate-mediated ROS homeostasis to promote rice thermoresponsive growth

© 2024 The Author(s). New Phytologist © 2024 New Phytologist Foundation.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 243(2024), 5 vom: 01. Aug., Seite 1742-1757
1. Verfasser: Li, Xueting (VerfasserIn)
Weitere Verfasser: Tang, Huashan, Xu, Ting, Wang, Pengfei, Ma, Fangfang, Wei, Haifang, Fang, Zi, Wu, Xiaoyan, Wang, Yanan, Xue, Yongbiao, Zhang, Biyao
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2024
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article N‐terminal acetylation ROS glycolate oxidase rice temperature thermoresponsive growth Reactive Oxygen Species Plant Proteins Glycolates mehr... glycolic acid 0WT12SX38S Hydrogen Peroxide BBX060AN9V glycollate oxidase EC 1.1.3.15 Alcohol Oxidoreductases EC 1.1.-
Beschreibung
Zusammenfassung:© 2024 The Author(s). New Phytologist © 2024 New Phytologist Foundation.
Climate warming poses a significant threat to global crop production and food security. However, our understanding of the molecular mechanisms governing thermoresponsive development in crops remains limited. Here we report that the auxiliary subunit of N-terminal acetyltransferase A (NatA) in rice OsNAA15 is a prerequisite for rice thermoresponsive growth. OsNAA15 produces two isoforms OsNAA15.1 and OsNAA15.2, via temperature-dependent alternative splicing. Among the two, OsNAA15.1 is more likely to form a stable and functional NatA complex with the potential catalytic subunit OsNAA10, leading to a thermoresponsive N-terminal acetylome. Intriguingly, while OsNAA15.1 promotes plant growth under elevated temperatures, OsNAA15.2 exhibits an inhibitory effect. We identified two glycolate oxidases (GLO1/5) as major substrates from the thermoresponsive acetylome. These enzymes are involved in hydrogen peroxide (H2O2) biosynthesis via glycolate oxidation. N-terminally acetylated GLO1/5 undergo their degradation through the ubiquitin-proteasome system. This leads to reduced reactive oxygen species (ROS) production, thereby promoting plant growth, particularly under high ambient temperatures. Conclusively, our findings highlight the pivotal role of N-terminal acetylation in orchestrating the glycolate-mediated ROS homeostasis to facilitate thermoresponsive growth in rice
Beschreibung:Date Completed 01.08.2024
Date Revised 01.08.2024
published: Print-Electronic
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.19928