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240616s2024 xx |||||o 00| ||eng c |
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|a 10.1016/j.plantsci.2024.112154
|2 doi
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|a pubmed24n1500.xml
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|a (PII)S0168-9452(24)00181-X
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|e rakwb
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|a eng
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| 100 |
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|a Chu, Wenlong
|e verfasserin
|4 aut
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| 245 |
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|a Cloning and functional verification of the male sterile gene BrQRT3 in Chinese cabbage
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|c 2024
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|a Date Completed 15.07.2024
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|a Date Revised 13.08.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2024 Elsevier B.V. All rights reserved.
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|a Chinese cabbage is a cross-pollinated crop with significant heterosis, and male sterile lines are an important way to produce hybrid seeds. In this study, a male sterile mutant msm0795 was identified in an EMS-mutagenized population of Chinese cabbage. Cytological observations revealed that the microspores failed to separate after the tetrad stage, and thus developed into abnormal pollen grains, resulting in anther abortion. MutMap combined with Kompetitive Allele Specific PCR genotyping showed that BraA01g011280.3.5 C was identified as the candidate gene, which encodes polygalacturonase QRT3 and plays a direct role in the degradation of pollen mother cell wall during microspore development, named BrQRT3. Subcellular localization and expression analyses demonstrated that BrQRT3 was localized in the cell membrane and was ubiquitously expressed in roots, stems, leaves, flower buds, and flowers, but the expression of BrQRT3 was gradually suppressed with the anther development. Ectopic expression confirmed that over-expression of BrQRT3 in qrt3 background Arabidopsis mutant can rescue the pollen defects caused by loss of AtQRT3 function. It is the first time to achieve a male sterile mutant caused by the mutation of BrQRT3 in Chinese cabbage. These findings contribute to elucidate the mechanism of BrQRT3 in regulating stamen development of Chinese cabbage
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|a Journal Article
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|a Chinese cabbage
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|a EMS mutagenesis
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|a Male sterility
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|a MutMap
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|a Polygalacturonase
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| 650 |
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|a Plant Proteins
|2 NLM
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| 700 |
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|a Dong, Shiyao
|e verfasserin
|4 aut
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| 700 |
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|a Zou, Jiaqi
|e verfasserin
|4 aut
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| 700 |
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|a Huang, Shengnan
|e verfasserin
|4 aut
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| 700 |
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|a Feng, Hui
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant science : an international journal of experimental plant biology
|d 1985
|g 346(2024) vom: 01. Aug., Seite 112154
|w (DE-627)NLM098174193
|x 1873-2259
|7 nnns
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|g volume:346
|g year:2024
|g day:01
|g month:08
|g pages:112154
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|u http://dx.doi.org/10.1016/j.plantsci.2024.112154
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