PsSOC1 is involved in the gibberellin pathway to trigger cell proliferation and budburst during endodormancy release in tree peony

PsSOC1 is involved in the gibberellin pathway to trigger cell proliferation and budburst during endodormancy release in tree peony

© 2024 The Authors. New Phytologist © 2024 New Phytologist Foundation.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 243(2024), 3 vom: 14. Juli, Seite 1017-1033
1. Verfasser: Zhang, Yuxi (VerfasserIn)
Weitere Verfasser: Niu, Demei, Yuan, Yanchao, Liu, Fang, Wang, Zhiwei, Gao, Linqiang, Liu, Chunying, Zhou, Gongke, Gai, Shupeng
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2024
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article PsCYCD PsRGL1 PsSOC1 cell proliferation endodormancy release tree peony Plant Proteins Gibberellins
Beschreibung
Zusammenfassung:© 2024 The Authors. New Phytologist © 2024 New Phytologist Foundation.
Tree peony (Paeonia suffruticosa) undergoes bud endodormancy, and gibberellin (GA) pathway plays a crucial role in dormancy regulation. Recently, a key DELLA protein PsRGL1 has been identified as a negative regulator of bud dormancy release. However, the mechanism of GA signal to break bud dormancy remains unknown. In this study, yeast two-hybrid screened PsSOC1 interacting with PsRGL1 through its MADS domain, and interaction was identified using pull-down and luciferase complementation imaging assays Transformation in tree peony and hybrid poplar confirmed that PsSOC1 facilitated bud dormancy release. Transcriptome analysis of PsSOC1-overexpressed buds indicated PsCYCD3.3 and PsEBB3 were its potential downstream targets combining with promoter survey, and they also accelerated bud dormancy release verified by genetic analysis. Yeast one-hybrid, electrophoretic mobility shifts assays, chromatin immunoprecipitation quantitative PCR, and dual luciferase assays confirmed that PsSOC1 could directly bind to the CArG motif of PsCYCD3.3 and PsEBB3 promoters via its MADS domain. PsRGL1-PsSOC1 interaction inhibited the DNA-binding activity of PsSOC1. Additionally, PsCYCD3.3 promoted bud dormancy release by rebooting cell proliferation. These findings elucidated a novel GA pathway, GA-PsRGL1-PsSOC1-PsCYCDs, which expanded our understanding of the GA pathway in bud dormancy release
Beschreibung:Date Completed 04.07.2024
Date Revised 09.07.2024
published: Print-Electronic
RefSeq: KF984039, OP095871, OP095872, OP095873, OP095874, OP734236, ADI46627.1, XP_002318176.3, XP_002298743.1
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.19893