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240615s2024 xx |||||o 00| ||eng c |
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|a 10.1111/nph.19902
|2 doi
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|a pubmed24n1476.xml
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|a (NLM)38877698
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
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|a Cao, Yimeng
|e verfasserin
|4 aut
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| 245 |
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|a The RXLR effector PpE18 of Phytophthora parasitica is a virulence factor and suppresses peroxisome membrane-associated ascorbate peroxidase NbAPX3-1-mediated plant immunity
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|c 2024
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 18.07.2024
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|a Date Revised 19.07.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2024 The Authors. New Phytologist © 2024 New Phytologist Foundation.
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|a Phytophthora parasitica causes diseases on a broad range of host plants. It secretes numerous effectors to suppress plant immunity. However, only a few virulence effectors in P. parasitica have been characterized. Here, we highlight that PpE18, a conserved RXLR effector in P. parasitica, was a virulence factor and suppresses Nicotiana benthamiana immunity. Utilizing luciferase complementation, co-immunoprecipitation, and GST pull-down assays, we determined that PpE18 targeted NbAPX3-1, a peroxisome membrane-associated ascorbate peroxidase with reactive oxygen species (ROS)-scavenging activity and positively regulates plant immunity in N. benthamiana. We show that the ROS-scavenging activity of NbAPX3-1 was critical for its immune function and was hindered by the binding of PpE18. The interaction between PpE18 and NbAPX3-1 resulted in an elevation of ROS levels in the peroxisome. Moreover, we discovered that the ankyrin repeat-containing protein NbANKr2 acted as a positive immune regulator, interacting with both NbAPX3-1 and PpE18. NbANKr2 was required for NbAPX3-1-mediated disease resistance. PpE18 competitively interfered with the interaction between NbAPX3-1 and NbANKr2, thereby weakening plant resistance. Our results reveal an effective counter-defense mechanism by which P. parasitica employed effector PpE18 to suppress host cellular defense, by suppressing biochemical activity and disturbing immune function of NbAPX3-1 during infection
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|a Journal Article
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|a Phytophthora parasitica
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| 650 |
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|a NbAPX3‐1
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|a PpE18
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4 |
|a ROS‐scavenging activity
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|a RXLR effector
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|a peroxisome
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4 |
|a plant immunity
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| 650 |
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|a Reactive Oxygen Species
|2 NLM
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| 650 |
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|a Ascorbate Peroxidases
|2 NLM
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| 650 |
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|a EC 1.11.1.11
|2 NLM
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| 650 |
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|a Virulence Factors
|2 NLM
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| 700 |
1 |
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|a Zhang, Qiang
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Liu, Yuan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yan, Tiantian
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ding, Liwen
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yang, Yang
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Meng, Yuling
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Shan, Weixing
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t The New phytologist
|d 1979
|g 243(2024), 4 vom: 14. Juli, Seite 1472-1489
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
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| 773 |
1 |
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|g volume:243
|g year:2024
|g number:4
|g day:14
|g month:07
|g pages:1472-1489
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| 856 |
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|u http://dx.doi.org/10.1111/nph.19902
|3 Volltext
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|d 243
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|h 1472-1489
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