Rice floury endosperm26 encoding a mitochondrial single-stranded DNA-binding protein is essential for RNA-splicing of mitochondrial genes and endosperm development

Bibliographische Detailangaben
Veröffentlicht in:	Plant science : an international journal of experimental plant biology. - 1985. - 346(2024) vom: 01. Aug., Seite 112151
1. Verfasser:	Teng, Xuan (VerfasserIn)
Weitere Verfasser:	Wang, Yongfei, Liu, Linglong, Yang, Hang, Wu, Mingming, Chen, Xiaoli, Ren, Yulong, Wang, Yunlong, Duan, Erchao, Dong, Hui, Jiang, Ling, Zhang, Yuanyan, Zhang, Wenwei, Chen, Rongbo, Liu, Shijia, Liu, Xi, Tian, Yunlu, Chen, Liangming, Wang, Yihua, Wan, Jianmin
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2024
Zugriff auf das übergeordnete Werk:	Plant science : an international journal of experimental plant biology
Schlagworte:	Journal Article Floury endosperm Mitochondrion Oryza sativa RNA splicing Single-stranded DNA-binding protein Plant Proteins DNA-Binding Proteins

Beschreibung
Zusammenfassung:	Copyright © 2024 Elsevier B.V. All rights reserved. Endosperm, the major storage organ in cereal grains, determines the grain yield and quality. Mitochondria provide the energy for dry matter accumulation, in the endosperm development. Although mitochondrial single-stranded DNA-binding proteins (mtSSBs) play a canonical role in the maintenance of single-stranded mitochondrial DNA, their molecular functions in RNA processing and endosperm development remain obscure. Here, we report a defective rice endosperm mutant, floury endosperm26 (flo26), which develops abnormal starch grains in the endosperm. Map-based cloning and complementation experiments showed that FLO26 allele encodes a mitochondrial single-stranded DNA-binding protein, named as mtSSB1.1. Loss of function of mtSSB1.1 affects the transcriptional level of many mitochondrially-encoded genes and RNA splicing of nad1, a core component of respiratory chain complex I in mitochondria. As a result, dysfunctional mature nad1 led to dramatically decreased complex I activity, thereby reducing ATP production. Our results reveal that mtSSB1.1 plays an important role in the maintenance of mitochondrial function and endosperm development by stabilizing the splicing of mitochondrial RNA in rice
Beschreibung:	Date Completed 15.07.2024 Date Revised 13.08.2024 published: Print-Electronic Citation Status MEDLINE
ISSN:	1873-2259
DOI:	10.1016/j.plantsci.2024.112151