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240522s2024 xx |||||o 00| ||eng c |
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|a 10.1107/S1600577524002923
|2 doi
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|a pubmed24n1462.xml
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|a (NLM)38771775
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Nikitin, Viktor
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Laminography as a tool for imaging large-size samples with high resolution
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|c 2024
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Revised 05.07.2024
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|a published: Print-Electronic
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|a Citation Status PubMed-not-MEDLINE
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|a open access.
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|a Despite the increased brilliance of the new generation synchrotron sources, there is still a challenge with high-resolution scanning of very thick and absorbing samples, such as a whole mouse brain stained with heavy elements, and, extending further, brains of primates. Samples are typically cut into smaller parts, to ensure a sufficient X-ray transmission, and scanned separately. Compared with the standard tomography setup where the sample would be cut into many pillars, the laminographic geometry operates with slab-shaped sections significantly reducing the number of sample parts to be prepared, the cutting damage and data stitching problems. In this work, a laminography pipeline for imaging large samples (>1 cm) at micrometre resolution is presented. The implementation includes a low-cost instrument setup installed at the 2-BM micro-CT beamline of the Advanced Photon Source. Additionally, sample mounting, scanning techniques, data stitching procedures, a fast reconstruction algorithm with low computational complexity, and accelerated reconstruction on multi-GPU systems for processing large-scale datasets are presented. The applicability of the whole laminography pipeline was demonstrated by imaging four sequential slabs throughout an entire mouse brain sample stained with osmium, in total generating approximately 12 TB of raw data for reconstruction
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|a Journal Article
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|a GPU
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|a laminography
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|a neuroimaging
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| 650 |
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|a reconstruction
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| 700 |
1 |
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|a Wildenberg, Gregg
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Mittone, Alberto
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Shevchenko, Pavel
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Deriy, Alex
|e verfasserin
|4 aut
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| 700 |
1 |
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|a De Carlo, Francesco
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Journal of synchrotron radiation
|d 1994
|g 31(2024), Pt 4 vom: 01. Juli, Seite 851-866
|w (DE-627)NLM09824129X
|x 1600-5775
|7 nnns
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| 773 |
1 |
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|g volume:31
|g year:2024
|g number:Pt 4
|g day:01
|g month:07
|g pages:851-866
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| 856 |
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|u http://dx.doi.org/10.1107/S1600577524002923
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|d 31
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|h 851-866
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