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240519s2024 xx |||||o 00| ||eng c |
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|a 10.1002/adma.202404607
|2 doi
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|a pubmed24n1488.xml
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|a eng
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| 100 |
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|a Ma, Huan
|e verfasserin
|4 aut
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| 245 |
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|a Protocell Flow Reactors for Enzyme and Whole-Cell Mediated Biocatalysis
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|c 2024
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|a Text
|b txt
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|a Date Completed 01.08.2024
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|a Date Revised 01.08.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2024 The Author(s). Advanced Materials published by Wiley‐VCH GmbH.
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|a The design and construction of continuous flow biochemical reactors comprising immobilized biocatalysts have generated great interest in the efficient synthesis of value-added chemicals. Living cells use compartmentalization and reaction-diffusion processes for spatiotemporal regulation of biocatalytic reactions, and implementing these strategies into continuous flow reactors can offer new opportunities in reactor design and application. Herein, the fabrication of protocell-based continuous flow reactors for enzyme and whole-cell mediated biocatalysis is demonstrated. Semipermeable membranized coacervate vesicles are employed as model protocells that spontaneously sequester enzymes or accumulate living bacteria to produce embodied microreactors capable of single- or multiple-step catalytic reactions. By packing millions of the enzyme/bacteria-containing coacervate vesicles in a glass column, a facile, cost-effective, and modular methodology capable of performing oxidoreductase, peroxidase and lipolytic reactions, enzyme-mediated L-DOPA synthesis, and whole-cell glycolysis under continuous flow conditions, is demonstrated. It is shown that the protocell-nested enzymes and bacterial cells exhibit enhanced activities and stability under deleterious operating conditions compared with their non-encapsulated counterparts. These results provide a step toward the engineering of continuous flow reactors based on cell-like microscale agents and offer opportunities in the development of green and sustainable industrial bioprocessing
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|a Journal Article
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|a coacervates
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|a continuous flow biocatalysis
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|a protocells
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|a Enzymes, Immobilized
|2 NLM
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|a Enzymes
|2 NLM
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1 |
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|a Liu, Xiayi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Nobbs, Angela H
|e verfasserin
|4 aut
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| 700 |
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|a Mishra, Ananya
|e verfasserin
|4 aut
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|a Patil, Avinash J
|e verfasserin
|4 aut
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| 700 |
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|a Mann, Stephen
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Advanced materials (Deerfield Beach, Fla.)
|d 1998
|g 36(2024), 31 vom: 31. Aug., Seite e2404607
|w (DE-627)NLM098206397
|x 1521-4095
|7 nnns
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|g volume:36
|g year:2024
|g number:31
|g day:31
|g month:08
|g pages:e2404607
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|u http://dx.doi.org/10.1002/adma.202404607
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