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|a 10.1021/acs.langmuir.4c00657
|2 doi
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|a eng
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| 100 |
1 |
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|a Zhang, Yaqin
|e verfasserin
|4 aut
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| 245 |
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|a Label-free Colorimetric Detection of Viral RNA Based on Clustered Regularly Interspaced Short Palindromic Repeats and Gold Nanoparticles with a Portable Device
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|c 2024
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| 336 |
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|a Text
|b txt
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 04.06.2024
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|a Date Revised 04.06.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Viral infections, such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), are some of the most dangerous threats to humans. SARS-CoV-2 has caused a global pandemic, highlighting the unprecedented demand for rapid and portable diagnostic methods. To meet these requirements, we designed a label-free colorimetric platform that combines the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated proteins (Cas) 12a system for naked-eye detection (named LFP). This method utilizes reverse transcription loop-mediated isothermal amplification (RT-LAMP) and the trans-cleavage activity of the CRISPR/Cas12a system to increase the sensitivity and specificity of the reaction. This platform can detect as few as 4 copies/μL of RNA and produces no false positive results when tested against the influenza virus. To better meet the requirements of point-of-care (POC) detection, we developed a portable device that can be applied in resource-poor and densely populated regions. The LFP assay holds great potential for application in resource-limited settings, and the label-free gold nanoparticle (AuNPs) probe can reduce costs, making it suitable for large-scale screening. We expect that the LFP assay will be promising for the POC screening of COVID-19
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|a Journal Article
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|a 7440-57-5
|2 NLM
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7 |
|a RNA, Viral
|2 NLM
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| 700 |
1 |
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|a Chen, Jianai
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kong, Fange
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Chunxia
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Guo, Hangyu
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Yingchun
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lu, Jiahui
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhang, Jicheng
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Jiasi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhou, Yulin
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1985
|g 40(2024), 22 vom: 04. Juni, Seite 11534-11540
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnas
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|g volume:40
|g year:2024
|g number:22
|g day:04
|g month:06
|g pages:11534-11540
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|u http://dx.doi.org/10.1021/acs.langmuir.4c00657
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