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240510s2024 xx |||||o 00| ||eng c |
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|a 10.1002/adma.202404901
|2 doi
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|a pubmed24n1482.xml
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|a (NLM)38723206
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|a DE-627
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Li, Feiyu
|e verfasserin
|4 aut
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| 245 |
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|a A Customized Biohybrid Presenting Cascade Responses to Tumor Microenvironment
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|c 2024
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 25.07.2024
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|a Date Revised 25.07.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2024 Wiley‐VCH GmbH.
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|a Intrinsic characteristics of microorganisms, including non-specific metabolism sites, toxic byproducts, and uncontrolled proliferation constrain their exploitation in medical applications such as tumor therapy. Here, the authors report an engineered biohybrid that can efficiently target cancerous sites through a pre-determined metabolic pathway to enable precise tumor ablation. In this system, DH5α Escherichia coli is engineered by the introduction of hypoxia-inducible promoters and lactate oxidase genes, and further surface-armored with iron-doped ZIF-8 nanoparticles. This bioengineered E. coli can produce and secrete lactate oxidase to reduce lactate concentration in response to hypoxic tumor microenvironment, as well as triggering immune activation. The peroxidase-like functionality of the nanoparticles extends the end product of the lactate metabolism, enabling the conversion of hydrogen peroxide (H2O2) into highly cytotoxic hydroxyl radicals. This, coupled with the transformation of tirapazamine loaded on nanoparticles to toxic benzotriazinyl, culminates in severe tumor cell ferroptosis. Intravenous injection of this biohybrid significantly inhibits tumor growth and metastasis
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|a Journal Article
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|a bacteria
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|a biohybrids
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|a engineering
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|a nanozymes
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|a tumor therapy
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|a lactate 2-monooxygenase
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|a EC 1.13.12.4
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|a Mixed Function Oxygenases
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|a EC 1.-
|2 NLM
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|a Tirapazamine
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|a 1UD32YR59G
|2 NLM
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|a Hydrogen Peroxide
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|a BBX060AN9V
|2 NLM
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|a ZIF-8 metal-organic framework
|2 NLM
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|a Metal-Organic Frameworks
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|a Imidazoles
|2 NLM
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|a Lactic Acid
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|a 33X04XA5AT
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|a Hydroxyl Radical
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|a 3352-57-6
|2 NLM
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|a Antineoplastic Agents
|2 NLM
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| 700 |
1 |
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|a Zhu, Peipei
|e verfasserin
|4 aut
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1 |
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|a Zheng, Bingzhu
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lu, Zijie
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Fang, Chao
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Fu, Yike
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Xiang
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Advanced materials (Deerfield Beach, Fla.)
|d 1998
|g 36(2024), 30 vom: 25. Juli, Seite e2404901
|w (DE-627)NLM098206397
|x 1521-4095
|7 nnns
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| 773 |
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|g volume:36
|g year:2024
|g number:30
|g day:25
|g month:07
|g pages:e2404901
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|u http://dx.doi.org/10.1002/adma.202404901
|3 Volltext
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