Evaluating UV-C Sensitivity of Calonectria pseudonaviculata in Model Buffer Solution Using a UV-C Light-Emitting-Diode System
Calonectria pseudonaviculata, responsible for boxwood blight, produces sticky conidia that pose a contamination risk in boxwood production via cross-contamination from tools, equipment, and other resources. This study evaluated UV-C light-emitting-diode (LED) irradiation (263 to 287 nm) as a disinfe...
| Veröffentlicht in: | Plant disease. - 1997. - 108(2024), 9 vom: 01. Sept., Seite 2663-2667 |
|---|---|
| 1. Verfasser: | |
| Weitere Verfasser: | , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2024
|
| Zugriff auf das übergeordnete Werk: | Plant disease |
| Schlagworte: | Journal Article D(10) values boxwood blight dose kinetics modeling |
| Zusammenfassung: | Calonectria pseudonaviculata, responsible for boxwood blight, produces sticky conidia that pose a contamination risk in boxwood production via cross-contamination from tools, equipment, and other resources. This study evaluated UV-C light-emitting-diode (LED) irradiation (263 to 287 nm) as a disinfection method by examining its effectiveness in inactivating conidia and determining the UV-C sensitivity. Conidial suspensions were exposed to quantifiable UV-C doses under a dynamic stirring condition. Average volumetric intensity was quantified by accounting for UV gradients and UV dose was calculated as a product of average fluence rate (mW⋅cm-2) and exposure time (s). UV-C irradiation effectively inactivated the tested pathogen following log-linear + shoulder kinetics as identified by parameters of goodness of model fit (i.e., high R2 and low root mean square error [RMSE] values). The model predicted the UV sensitivity of C. pseudonaviculata conidia as 46.6 mJ⋅cm-2 per log. A total of 2.04 log reductions of the population could be obtained by an exposure of 60 mJ⋅cm-2 of UV-C dose. The calculated decimal reduction dose (D10) was 13.53 ± 0.98 mJ⋅cm-2 (R2 = 0.97, RMSE = 0.14), inactivation rate constant (Kmax) = 0.17 ± 0.01, and shoulder length = 33.06 ± 1.81 mJ⋅cm-2. These findings indicate that UV-C irradiation could be a viable option for disinfecting tools, equipment, and possibly propagation cuttings in nurseries |
|---|---|
| Beschreibung: | Date Completed 24.09.2024 Date Revised 24.09.2024 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 0191-2917 |
| DOI: | 10.1094/PDIS-03-24-0618-SC |