Systemic long-distance sulfur transport and its role in symbiotic root nodule protein turnover

Copyright © 2024 The Authors. Published by Elsevier GmbH.. All rights reserved.

Détails bibliographiques
Publié dans:Journal of plant physiology. - 1979. - 297(2024) vom: 16. Juni, Seite 154260
Auteur principal: Siegl, Alina (Auteur)
Autres auteurs: Afjehi-Sadat, Leila, Wienkoop, Stefanie
Format: Article en ligne
Langue:English
Publié: 2024
Accès à la collection:Journal of plant physiology
Sujets:Journal Article Rhizobium symbiosis Root nodule proteome Split-root-system Stable isotope labeling Sulfate transport Systemic long-distance transport
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520 |a Sulfur is an essential nutrient for all plants, but also crucial for the nitrogen fixing symbiosis between legumes and rhizobia. Sulfur limitation can hamper nodule development and functioning. Until now, it remained unclear whether sulfate uptake into nodules is local or mainly systemic via the roots, and if long-distance transport from shoots to roots and into nodules occurs. Therefore, this work investigates the systemic regulation of sulfur transportation in the model legume Lotus japonicus by applying stable isotope labeling to a split-root system. Metabolite and protein extraction together with mass spectrometry analyses were conducted to determine the plants molecular phenotype and relative isotope protein abundances. Data show that treatments of varying sulfate concentrations including the absence of sulfate on one side of a nodulated root was not affecting nodule development as long as the other side of the root system was provided with sufficient sulfate. Concentrations of shoot metabolites did not indicate a significant stress response caused by a lack of sulfur. Further, we did not observe any quantitative changes in proteins involved in biological nitrogen fixation in response to the different sulfate treatments. Relative isotope abundance of 34S confirmed a long-distance transport of sulfur from one side of the roots to the other side and into the nodules. Altogether, these results provide evidence for a systemic long-distance transport of sulfur via the upper part of the plant to the nodules suggesting a demand driven sulfur distribution for the maintenance of symbiotic N-fixation 
650 4 |a Journal Article 
650 4 |a Rhizobium symbiosis 
650 4 |a Root nodule proteome 
650 4 |a Split-root-system 
650 4 |a Stable isotope labeling 
650 4 |a Sulfate transport 
650 4 |a Systemic long-distance transport 
700 1 |a Afjehi-Sadat, Leila  |e verfasserin  |4 aut 
700 1 |a Wienkoop, Stefanie  |e verfasserin  |4 aut 
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