The nematode effector Mj-NEROSs interacts with Rieske's iron-sulfur protein influencing plastid ROS production to suppress plant immunity

Bibliographische Detailangaben
Veröffentlicht in:	The New phytologist. - 1979. - 242(2024), 6 vom: 25. Juni, Seite 2787-2802
1. Verfasser:	Stojilković, Boris (VerfasserIn)
Weitere Verfasser:	Xiang, Hui, Chen, Yujin, Maulana, Muhammad Iqbal, Bauters, Lander, Van de Put, Hans, Steppe, Kathy, Liao, Jinling, de Almeida Engler, Janice, Gheysen, Godelieve
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2024
Zugriff auf das übergeordnete Werk:	The New phytologist
Schlagworte:	Journal Article ISP ROS effector plant defense protein–protein interaction root plastids Arabidopsis Proteins Electron Transport Complex III EC 7.1.1.8 mehr... Helminth Proteins Iron-Sulfur Proteins Reactive Oxygen Species Rieske iron-sulfur protein

Beschreibung
Zusammenfassung:	© 2024 The Authors New Phytologist © 2024 New Phytologist Foundation. Root-knot nematodes (RKN; Meloidogyne species) are plant pathogens that introduce several effectors in their hosts to facilitate infection. The actual targets and functioning mechanism of these effectors largely remain unexplored. This study illuminates the role and interplay of the Meloidogyne javanica nematode effector ROS suppressor (Mj-NEROSs) within the host plant environment. Mj-NEROSs suppresses INF1-induced cell death as well as flg22-induced callose deposition and reactive oxygen species (ROS) production. A transcriptome analysis highlighted the downregulation of ROS-related genes upon Mj-NEROSs expression. NEROSs interacts with the plant Rieske's iron-sulfur protein (ISP) as shown by yeast-two-hybrid and bimolecular fluorescence complementation. Secreted from the subventral pharyngeal glands into giant cells, Mj-NEROSs localizes in the plastids where it interacts with ISP, subsequently altering electron transport rates and ROS production. Moreover, our results demonstrate that isp Arabidopsis thaliana mutants exhibit increased susceptibility to M. javanica, indicating ISP importance for plant immunity. The interaction of a nematode effector with a plastid protein highlights the possible role of root plastids in plant defense, prompting many questions on the details of this process
Beschreibung:	Date Completed 23.05.2024 Date Revised 29.07.2024 published: Print-Electronic RefSeq: AF531162 Citation Status MEDLINE
ISSN:	1469-8137
DOI:	10.1111/nph.19781