'Gel-Stacks' gently confine or reversibly immobilize arrays of single DNA molecules for manipulation and study

Large DNA molecules (>20 kb) are difficult analytes prone to breakage during serial manipulations and cannot be 'rescued' as full-length amplicons. Accordingly, to present, modify and analyze arrays of large, single DNA molecules, we created an easily realizable approach offering gentle...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1988. - 76(2024), 6 vom: 24., Seite 285-289
1. Verfasser: Calle-Casteñeda, Susana (VerfasserIn)
Weitere Verfasser: Winden, Eamon, Vasquez-Echeverri, Alejandro, Schickling, Matthew, Browning, Evelyn, Hernandez Ortiz, Juan Pablo, Schwartz, David C
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2024
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Fick's second law confinement epifluorescence microscopy hydrogels large DNA molecule arrays reagent transport DNA 9007-49-2 Hydrogels mehr... Immobilized Nucleic Acids Acrylic Resins Spermidine U87FK77H25