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|a 10.1094/PDIS-09-23-1890-PDN
|2 doi
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|a eng
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|a Song, Yunze
|e verfasserin
|4 aut
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|a First Report of Leaf Rot Caused by Apiospora paraphaeosperma on Lily in Wuhan, China
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|c 2024
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|a Text
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|a Date Revised 24.04.2024
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|a published: Print-Electronic
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|a Citation Status Publisher
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|a Lily (Lilium spp.) is a valuable ornamental bulb flower plant in Liliaceae, and its bulbs have high edible and medicinal value. Compared with bulb propagation of other lilies, seed propagation and short growth period are the most significant characteristics of Lilium×formolongi. In 2023, leaf rot disease (LRD) was observed on approximately 70% of the Lilium×formolongi seedlings sown in an experimental greenhouse in Wuhan, Hubei province, China. Irregular brown water-soaked spots were discovered in the early stages of infected seedlings. Then, spots spread throughout the leaves and caused the leaves to brown, soften, and wilted. A pathogen associated with symptoms was isolated by incubating sterilized leaves on potato dextrose agar plates at 25 ℃ for 2-3 days. Then, a pure single colony was isolated through a single hyphal tip isolation method. The fungal colony was white with abundant aerial mycelium and produced a yellow pigment diffusible into the agar. Microscopically, isolated mycelia were reticulate and pale yellow, while conidia were dark brown, smooth, and spherical, 7.31 to 6.98 × 4.03 to 3.87μm (average 5.44×5.41μm; n=30); oval in lateral view, and had a light stripe in the middle. To identify the species of the fungus at the molecular level, ITS and EF-1α genes were amplified and sequenced using primers ITS1/ITS4 (M Gardes et al. 1993) and 758F/986R (Carbone and Kohn 1999). The BLAST results in GenBank showed that the ITS(OR523578) and EF-1α(PP066842) sequences of LRD shared 99.82% and 99.24% identity with the distinct Apiospora paraphaeosperma strains (GenBank accession MT040110, ON806628.1, respectively). Combined with the morphology of the colony and conidium, the fungus was identified as Ap. paraphaeosperma. In the pathogenicity test, six healthy leaves were inoculated with mycelium disc and then kept in an incubator (22 ℃, 90% humidity, 16h light /8h darkness). The inoculated leaves showed necrosis and wilt symptoms similar to those observed in the greenhouse, while the control leaves were asymptomatic. A re-isolation, morphology identification and DNA sequencing of the fungus confirmed its infection with Ap. paraphaeosperma in Lilium spp. At present, rot caused by Ap. paraphaeosperma has only been reported in Thailand and South Korea, both of which are found on bamboo stems (Hyde et al. 2016; Sun Lul Kwon et al. 2022). As far as we know, this is the first report of leaf rot of lily caused by Ap. paraphaeosperma in China. This report can help identify this disease and further develop effective control measures
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|a Journal Article
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|a Apiospora paraphaeosperma
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| 650 |
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|a Leaf Rot
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| 650 |
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|a Lily
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| 700 |
1 |
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|a Zhang, Lixue
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Huo, Xinyi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Yanpin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chen, Xiaolin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ning, GuoGui
|e verfasserin
|4 aut
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| 700 |
1 |
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|a He, Yanhong
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
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|u http://dx.doi.org/10.1094/PDIS-09-23-1890-PDN
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