Improving transformation and regeneration efficiency in medicinal plants : insights from other recalcitrant species
© The Author(s) 2024. Published by Oxford University Press on behalf of the Society for Experimental Biology.
| Veröffentlicht in: | Journal of experimental botany. - 1985. - 76(2024), 1 vom: 01. Jan., Seite 52-75 |
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| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2025
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| Zugriff auf das übergeordnete Werk: | Journal of experimental botany |
| Schlagworte: | Journal Article Review Explants medicinal plants morphogenic genes nanoparticles plant growth regulators recalcitrance regeneration transformation |
| Zusammenfassung: | © The Author(s) 2024. Published by Oxford University Press on behalf of the Society for Experimental Biology. Medicinal plants are integral to traditional medicine systems worldwide, being pivotal for human health. Harvesting plant material from natural environments, however, has led to species scarcity, prompting action to develop cultivation solutions that also aid conservation efforts. Biotechnological tools, specifically plant tissue culture and genetic transformation, offer solutions for sustainable, large-scale production and enhanced yield of valuable biomolecules. While these techniques are instrumental to the development of the medicinal plant industry, the challenge of inherent regeneration recalcitrance in some species to in vitro cultivation hampers these efforts. This review examines the strategies for overcoming recalcitrance in medicinal plants using a holistic approach, emphasizing the meticulous choice of explants (e.g. embryonic/meristematic tissues), plant growth regulators (e.g. synthetic cytokinins), and use of novel regeneration-enabling methods to deliver morphogenic genes (e.g. GRF/GIF chimeras and nanoparticles), which have been shown to contribute to overcoming recalcitrance barriers in agriculture crops. Furthermore, it highlights the benefit of cost-effective genomic technologies that enable precise genome editing and the value of integrating data-driven models to address genotype-specific challenges in medicinal plant research. These advances mark a progressive step towards a future where medicinal plant cultivation is not only more efficient and predictable but also inherently sustainable, ensuring the continued availability and exploitation of these important plants for current and future generations |
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| Beschreibung: | Date Completed 20.12.2024 Date Revised 20.12.2024 published: Print Citation Status MEDLINE |
| ISSN: | 1460-2431 |
| DOI: | 10.1093/jxb/erae189 |