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20240729232446.0 |
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240424s2024 xx |||||o 00| ||eng c |
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|a 10.1111/nph.19770
|2 doi
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|a pubmed24n1485.xml
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|a (DE-627)NLM371387078
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|a (NLM)38649769
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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1 |
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|a Chakraborty, Anirban
|e verfasserin
|4 aut
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| 245 |
1 |
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|a MicroRNA164e suppresses NAC100 transcription factor-mediated synthesis of seed storage proteins in chickpea
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|c 2024
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 23.05.2024
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|a Date Revised 29.07.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2024 The Authors New Phytologist © 2024 New Phytologist Foundation.
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|a Development of protein-enriched chickpea varieties necessitates an understanding of specific genes and key regulatory circuits that govern the synthesis of seed storage proteins (SSPs). Here, we demonstrated the novel involvement of Ca-miR164e-CaNAC100 in regulating SSP synthesis in chickpea. Ca-miRNA164e was significantly decreased during seed maturation, especially in high-protein accessions. The miRNA was found to directly target the transactivation conferring C-terminal region of a nuclear-localized transcription factor, CaNAC100 as revealed using RNA ligase-mediated-rapid amplification of cDNA ends and target mimic assays. The functional role of CaNAC100 was demonstrated through seed-specific overexpression (NACOE) resulting in significantly augmented seed protein content (SPC) consequential to increased SSP transcription. Further, NACOE lines displayed conspicuously enhanced seed weight but reduced numbers and yield. Conversely, a downregulation of CaNAC100 and SSP transcripts was evident in seed-specific overexpression lines of Ca-miR164e that culminated in significantly lowered SPC. CaNAC100 was additionally demonstrated to transactivate the SSP-encoding genes by directly binding to their promoters as demonstrated using electrophoretic mobility shift and dual-luciferase reporter assays. Taken together, our study for the first time established a distinct role of CaNAC100 in positively influencing SSP synthesis and its critical regulation by CamiR164e, thereby serving as an understanding that can be utilized for developing SPC-rich chickpea varieties
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|a Journal Article
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|a NAC100
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|a chickpea
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|a microRNA164
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4 |
|a seed protein content
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| 650 |
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4 |
|a seed storage proteins
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| 650 |
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4 |
|a transactivation
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| 650 |
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7 |
|a MicroRNAs
|2 NLM
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| 650 |
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7 |
|a Plant Proteins
|2 NLM
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| 650 |
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|a Seed Storage Proteins
|2 NLM
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| 650 |
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|a Transcription Factors
|2 NLM
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| 700 |
1 |
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|a Singh, Baljinder
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Pandey, Vimal
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Parida, Swarup K
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Bhatia, Sabhyata
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t The New phytologist
|d 1979
|g 242(2024), 6 vom: 12. Juni, Seite 2652-2668
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
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| 773 |
1 |
8 |
|g volume:242
|g year:2024
|g number:6
|g day:12
|g month:06
|g pages:2652-2668
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| 856 |
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|u http://dx.doi.org/10.1111/nph.19770
|3 Volltext
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