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240421s2024 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/erae180
|2 doi
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|a pubmed24n1529.xml
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|a (DE-627)NLM371310784
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|a (NLM)38642102
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
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|a Huang, Jinghua
|e verfasserin
|4 aut
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| 245 |
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|a OsRH52A, a DEAD-box protein, regulates functional megaspore specification and is required for embryo sac development in rice
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|c 2024
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 28.08.2024
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|a Date Revised 09.09.2024
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|a published: Print
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|a Citation Status MEDLINE
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|a © The Author(s) 2024. Published by Oxford University Press on behalf of the Society for Experimental Biology.
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|a The development of the embryo sac is an important factor that affects seed setting in rice. Numerous genes associated with embryo sac (ES) development have been identified in plants; however, the function of the DEAD-box RNA helicase family genes is poorly known in rice. Here, we characterized a rice DEAD-box protein, RH52A, which is localized in the nucleus and cytoplasm and highly expressed in the floral organs. The knockout mutant rh52a displayed partial ES sterility, including degeneration of the ES (21%) and the presence of a double-female-gametophyte (DFG) structure (11.8%). The DFG developed from two functional megaspores near the chalazal end in one ovule, and 3.4% of DFGs were able to fertilize via the sac near the micropylar pole in rh52a. RH52A was found to interact with MFS1 and ZIP4, both of which play a role in homologous recombination in rice meiosis. RNA-sequencing identified 234 down-regulated differentially expressed genes associated with reproductive development, including two, MSP1 and HSA1b, required for female germline cell specification. Taken together, our study demonstrates that RH52A is essential for the development of the rice embryo sac and provides cytological details regarding the formation of DFGs
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|a Journal Article
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|a Oryza sativa
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|a Cell specification
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|a DEAD-box RNA helicase
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|a chalazal functional megaspore
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|a double-female-gametophyte
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|a embryo sac
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|a rice
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| 650 |
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|a Plant Proteins
|2 NLM
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| 650 |
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|a DEAD-box RNA Helicases
|2 NLM
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| 650 |
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7 |
|a EC 3.6.4.13
|2 NLM
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| 700 |
1 |
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|a Qiao, Zhengping
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yu, Hang
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lu, Zijun
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chen, Weibin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lu, Junming
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wu, Jinwen
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Bao, Yueming
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Shahid, Muhammad Qasim
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Liu, Xiangdong
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 75(2024), 16 vom: 28. Aug., Seite 4802-4821
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
1 |
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|g volume:75
|g year:2024
|g number:16
|g day:28
|g month:08
|g pages:4802-4821
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| 856 |
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|u http://dx.doi.org/10.1093/jxb/erae180
|3 Volltext
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|d 75
|j 2024
|e 16
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|c 08
|h 4802-4821
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