Isotopically nonstationary metabolic flux analysis of plants : recent progress and future opportunities
© 2024 The Authors. New Phytologist © 2024 New Phytologist Foundation. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.
| Veröffentlicht in: | The New phytologist. - 1979. - 242(2024), 5 vom: 05. Juni, Seite 1911-1918 |
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| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2024
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| Zugriff auf das übergeordnete Werk: | The New phytologist |
| Schlagworte: | Journal Article Review Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. biochemical pathways carbon partitioning central carbon metabolism isotopic labeling metabolic flux analysis metabolic modeling mehr... |
| Zusammenfassung: | © 2024 The Authors. New Phytologist © 2024 New Phytologist Foundation. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA. Metabolic flux analysis (MFA) is a valuable tool for quantifying cellular phenotypes and to guide plant metabolic engineering. By introducing stable isotopic tracers and employing mathematical models, MFA can quantify the rates of metabolic reactions through biochemical pathways. Recent applications of isotopically nonstationary MFA (INST-MFA) to plants have elucidated nonintuitive metabolism in leaves under optimal and stress conditions, described coupled fluxes for fast-growing algae, and produced a synergistic multi-organ flux map that is a first in MFA for any biological system. These insights could not be elucidated through other approaches and show the potential of INST-MFA to correct an oversimplified understanding of plant metabolism |
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| Beschreibung: | Date Completed 08.05.2024 Date Revised 31.12.2024 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1469-8137 |
| DOI: | 10.1111/nph.19734 |