CPK10 protein kinase regulates Arabidopsis tolerance to boron deficiency through phosphorylation and activation of BOR1 transporter

CPK10 protein kinase regulates Arabidopsis tolerance to boron deficiency through phosphorylation and activation of BOR1 transporter

© 2024 The Author(s). New Phytologist © 2024 New Phytologist Foundation.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 243(2024), 5 vom: 01. Sept., Seite 1795-1809
1. Verfasser: Wang, Zhangqing (VerfasserIn)
Weitere Verfasser: Zhang, Yanting, Wu, Yaru, Lai, Duoduo, Deng, Yuan, Ju, Chuanfeng, Sun, Lv, Huang, Panpan, Wang, Cun
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2024
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Arabidopsis BOR1 Boron CPK10 Ca2+ signaling Antiporters Arabidopsis Proteins BOR1 protein, Arabidopsis N9E3X5056Q mehr... calcium-dependent protein kinase EC 2.7.1.- Protein Kinases EC 2.7.- ATCDPK1 protein, Arabidopsis
Beschreibung
Zusammenfassung:© 2024 The Author(s). New Phytologist © 2024 New Phytologist Foundation.
Boron (B) is crucial for plant growth and development. B deficiency can impair numerous physiological and metabolic processes, particularly in root development and pollen germination, seriously impeding crop growth and yield. However, the molecular mechanism underlying boron signal perception and signal transduction is rather limited. In this study, we discovered that CPK10, a calcium-dependent protein kinase in the CPK family, has the strongest interaction with the boron transporter BOR1. Mutations in CPK10 led to growth and root development defects under B-deficiency conditions, while constitutively active CPK10 enhanced plant tolerance to B deficiency. Furthermore, we found that CPK10 interacted with and phosphorylated BOR1 at the Ser689 residue. Through various biochemical analyses and complementation of B transport in yeast and plants, we revealed that Ser689 of BOR1 is important for its transport activity. In summary, these findings highlight the significance of the CPK10-BOR1 signaling pathway in maintaining B homeostasis in plants and provide targets for the genetic improvement of crop tolerance to B-deficiency stress
Beschreibung:Date Completed 01.08.2024
Date Revised 10.09.2024
published: Print-Electronic
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.19712