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240415s2024 xx |||||o 00| ||eng c |
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|a 10.1094/PDIS-06-23-1114-SR
|2 doi
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|a pubmed24n1548.xml
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|a (DE-627)NLM371057116
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|a (NLM)38616393
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Seress, Diána
|e verfasserin
|4 aut
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| 245 |
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|a Development and Implementation of a Novel CAPS Assay Reveals High Prevalence of a Boscalid Resistance Marker and Its Co-Occurrence with an Azole Resistance Marker in Erysiphe necator
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|c 2024
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 24.09.2024
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|a Date Revised 24.09.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Succinate dehydrogenase inhibitors (SDHIs) are frequently used against powdery mildew (PM) fungi, such as Erysiphe necator, the causal agent of grapevine PM. Fungicide resistance, however, hinders effective control. DNA-based monitoring facilitates the recognition of resistance. We aimed (i) to adapt an effective method to detect a widespread genetic marker of resistance to boscalid, a commonly used SDHI, and (ii) to study the co-occurrence of the marker with a marker of resistance to demethylase inhibitor (DMI) fungicides. Sequencing of the sdhB gene identified a nonsynonymous substitution, denoted as sdhB-A794G, leading to an amino acid change (H242R) in the sdhB protein. In vitro fungicide resistance tests showed that E. necator isolates carrying sdhB-A794G were resistant to boscalid. We adopted a cleaved amplified polymorphic sequence-based method and screened more than 500 field samples collected from five Hungarian wine regions in two consecutive years. The sdhB-A794G marker was detected in all wine regions and in both years, altogether in 61.7% of samples, including 20.5% in which both sdhB-A794G and the wild-type were present. The frequency of sdhB-A794G was higher in SDHI-treated vineyards than in vineyards without any SDHI application. A significant difference in the presence of the marker was detected among wine regions; its prevalence ranged from none to 100%. We identified significant co-occurrence of sdhB-A794G with the CYP51-A495T (Y136F) mutation of the CYP51 gene, a known marker of resistance to DMIs. The monitoring of fungicide resistance is fundamental for the successful control of E. necator. Our rapid, cost-effective diagnostic method will support decision-making and fungicide resistance monitoring and management
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|a Journal Article
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|a cleaved amplified polymorphic sequence
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|a diagnostics
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|a fungicide resistance management
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|a grapevine powdery mildew
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|a succinate dehydrogenase inhibitor
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|a 2-chloro-N-(4-chlorobiphenyl-2-yl)nicotinamide
|2 NLM
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|a 32MS8ZRD1V
|2 NLM
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| 650 |
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|a Fungicides, Industrial
|2 NLM
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| 650 |
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|a Niacinamide
|2 NLM
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| 650 |
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|a 25X51I8RD4
|2 NLM
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| 650 |
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|a Genetic Markers
|2 NLM
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| 650 |
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|a Azoles
|2 NLM
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| 650 |
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|a Succinate Dehydrogenase
|2 NLM
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| 650 |
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|a EC 1.3.99.1
|2 NLM
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| 650 |
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|a Fungal Proteins
|2 NLM
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| 650 |
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|a Biphenyl Compounds
|2 NLM
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| 700 |
1 |
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|a Molnár, Orsolya
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Matolcsi, Fruzsina
|e verfasserin
|4 aut
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| 700 |
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|a Pintye, Alexandra
|e verfasserin
|4 aut
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| 700 |
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|a Kovács, Gábor M
|e verfasserin
|4 aut
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| 700 |
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|a Németh, Márk Z
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant disease
|d 1997
|g 108(2024), 9 vom: 25. Sept., Seite 2607-2614
|w (DE-627)NLM098181742
|x 0191-2917
|7 nnns
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| 773 |
1 |
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|g volume:108
|g year:2024
|g number:9
|g day:25
|g month:09
|g pages:2607-2614
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| 856 |
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|u http://dx.doi.org/10.1094/PDIS-06-23-1114-SR
|3 Volltext
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|a AR
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|d 108
|j 2024
|e 9
|b 25
|c 09
|h 2607-2614
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