Cotton plants overexpressing the Bacillus thuringiensis Cry23Aa and Cry37Aa binary-like toxins exhibit high resistance to the cotton boll weevil (Anthonomus grandis)

Copyright © 2024 Elsevier B.V. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant science : an international journal of experimental plant biology. - 1985. - 344(2024) vom: 01. Mai, Seite 112079
1. Verfasser: Ribeiro, Thuanne Pires (VerfasserIn)
Weitere Verfasser: Martins-de-Sa, Diogo, Macedo, Leonardo Lima Pepino, Lourenço-Tessutti, Isabela Tristan, Ruffo, Gustavo Caseca, Sousa, João Pedro Abreu, Rósario Santana, Julia Moura do, Oliveira-Neto, Osmundo Brilhante, Moura, Stéfanie Menezes, Silva, Maria Cristina Mattar, Morgante, Carolina Vianna, Oliveira, Nelson Geraldo, Basso, Marcos Fernando, Grossi-de-Sa, Maria Fatima
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2024
Zugriff auf das übergeordnete Werk:Plant science : an international journal of experimental plant biology
Schlagworte:Journal Article Coleoptera Cry23Aa/Mpp23Aa Cry37Aa/Xpp37Aa Gossypium hirsutum Insect pest Insecticidal toxins Transgenic cotton insecticidal crystal protein, Bacillus Thuringiensis
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520 |a The cotton boll weevil (CBW, Anthonomus grandis) stands as one of the most significant threats to cotton crops (Gossypium hirsutum). Despite substantial efforts, the development of a commercially viable transgenic cotton event for effective open-field control of CBW has remained elusive. This study describes a detailed characterization of the insecticidal toxins Cry23Aa and Cry37Aa against CBW. Our findings reveal that CBW larvae fed on artificial diets supplemented exclusively with Cry23Aa decreased larval survival by roughly by 69%, while supplementation with Cry37Aa alone displayed no statistical difference compared to the control. However, the combined provision of both toxins in the artificial diet led to mortality rates approaching 100% among CBW larvae (LC50 equal to 0.26 PPM). Additionally, we engineered transgenic cotton plants by introducing cry23Aa and cry37Aa genes under control of the flower bud-specific pGhFS4 and pGhFS1 promoters, respectively. Seven transgenic cotton events expressing high levels of Cry23Aa and Cry37Aa toxins in flower buds were selected for greenhouse bioassays, and the mortality rate of CBW larvae feeding on their T0 and T1 generations ranged from 75% to 100%. Our in silico analyses unveiled that Cry23Aa displays all the hallmark characteristics of β-pore-forming toxins (β-PFTs) that bind to sugar moieties in glycoproteins. Intriguingly, we also discovered a distinctive zinc-binding site within Cry23Aa, which appears to be involved in protein-protein interactions. Finally, we discuss the major structural features of Cry23Aa that likely play a role in the toxin's mechanism of action. In view of the low LC50 for CBW larvae and the significant accumulation of these toxins in the flower buds of both T0 and T1 plants, we anticipate that through successive generations of these transgenic lines, cotton plants engineered to overexpress cry23Aa and cry37Aa hold promise for effectively managing CBW infestations in cotton crops 
650 4 |a Journal Article 
650 4 |a Coleoptera 
650 4 |a Cry23Aa/Mpp23Aa 
650 4 |a Cry37Aa/Xpp37Aa 
650 4 |a Gossypium hirsutum 
650 4 |a Insect pest 
650 4 |a Insecticidal toxins 
650 4 |a Transgenic cotton 
650 7 |a insecticidal crystal protein, Bacillus Thuringiensis  |2 NLM 
700 1 |a Martins-de-Sa, Diogo  |e verfasserin  |4 aut 
700 1 |a Macedo, Leonardo Lima Pepino  |e verfasserin  |4 aut 
700 1 |a Lourenço-Tessutti, Isabela Tristan  |e verfasserin  |4 aut 
700 1 |a Ruffo, Gustavo Caseca  |e verfasserin  |4 aut 
700 1 |a Sousa, João Pedro Abreu  |e verfasserin  |4 aut 
700 1 |a Rósario Santana, Julia Moura do  |e verfasserin  |4 aut 
700 1 |a Oliveira-Neto, Osmundo Brilhante  |e verfasserin  |4 aut 
700 1 |a Moura, Stéfanie Menezes  |e verfasserin  |4 aut 
700 1 |a Silva, Maria Cristina Mattar  |e verfasserin  |4 aut 
700 1 |a Morgante, Carolina Vianna  |e verfasserin  |4 aut 
700 1 |a Oliveira, Nelson Geraldo  |e verfasserin  |4 aut 
700 1 |a Basso, Marcos Fernando  |e verfasserin  |4 aut 
700 1 |a Grossi-de-Sa, Maria Fatima  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Plant science : an international journal of experimental plant biology  |d 1985  |g 344(2024) vom: 01. Mai, Seite 112079  |w (DE-627)NLM098174193  |x 1873-2259  |7 nnns 
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