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240405s2024 xx |||||o 00| ||eng c |
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|a 10.2144/btn-2023-0109
|2 doi
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|e rakwb
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|a eng
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1 |
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|a Leblanc, Julien
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Fully in vitro iterative construction of a 24 kb-long artificial DNA sequence to store digital information
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|c 2024
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 26.04.2024
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|a Date Revised 26.04.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a In the absence of a DNA template, the ab initio production of long double-stranded DNA molecules of predefined sequences is particularly challenging. The DNA synthesis step remains a bottleneck for many applications such as functional assessment of ancestral genes, analysis of alternative splicing or DNA-based data storage. In this report we propose a fully in vitro protocol to generate very long double-stranded DNA molecules starting from commercially available short DNA blocks in less than 3 days using Golden Gate assembly. This innovative application allowed us to streamline the process to produce a 24 kb-long DNA molecule storing part of the Declaration of the Rights of Man and of the Citizen of 1789 . The DNA molecule produced can be readily cloned into a suitable host/vector system for amplification and selection
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a DNA assembly
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|a DNA storage
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|a Oxford nanopore technology
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| 650 |
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|a long read sequencing
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|a synthetic DNA
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|a DNA
|2 NLM
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| 650 |
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|a 9007-49-2
|2 NLM
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| 700 |
1 |
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|a Boulle, Olivier
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Roux, Emeline
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Nicolas, Jacques
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lavenier, Dominique
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Audic, Yann
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1993
|g 76(2024), 5 vom: 19. Mai, Seite 203-215
|w (DE-627)NLM012627046
|x 1940-9818
|7 nnas
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| 773 |
1 |
8 |
|g volume:76
|g year:2024
|g number:5
|g day:19
|g month:05
|g pages:203-215
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| 856 |
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|u http://dx.doi.org/10.2144/btn-2023-0109
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