Fully in vitro iterative construction of a 24 kb-long artificial DNA sequence to store digital information
© 2024 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group
| Publié dans: | BioTechniques. - 1991. - 76(2024), 5 vom: 30. Mai, Seite 203-215 |
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| Auteur principal: | |
| Autres auteurs: | , , , , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2024
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| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article Research Support, Non-U.S. Gov't DNA assembly DNA storage Oxford nanopore technology long read sequencing synthetic DNA DNA 9007-49-2 |
| Résumé: | © 2024 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group In the absence of a DNA template, the ab initio production of long double-stranded DNA molecules of predefined sequences is particularly challenging. The DNA synthesis step remains a bottleneck for many applications such as functional assessment of ancestral genes, analysis of alternative splicing or DNA-based data storage. In this report we propose a fully in vitro protocol to generate very long double-stranded DNA molecules starting from commercially available short DNA blocks in less than 3 days using Golden Gate assembly. This innovative application allowed us to streamline the process to produce a 24 kb-long DNA molecule storing part of the Declaration of the Rights of Man and of the Citizen of 1789 . The DNA molecule produced can be readily cloned into a suitable host/vector system for amplification and selection |
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| Description: | Date Completed 26.04.2024 Date Revised 04.08.2025 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1940-9818 |
| DOI: | 10.2144/btn-2023-0109 |