Isothermal amplification of long DNA fragments at low temperature by improved strand displacement amplification

Strand displacement amplification (SDA) is an isothermal amplification technique wherein amplification of a nucleic acid is initiated by nicking enzyme activity at sites flanking the target. Diagnostic SDA is very fast but requires precise optimization and is limited to very short amplicons. Here we...

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Veröffentlicht in:BioTechniques. - 1988. - 76(2024), 6 vom: 28., Seite 255-262
1. Verfasser: Zhang, Yinhua (VerfasserIn)
Weitere Verfasser: Luck, Ashley N, Tanner, Nathan A
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2024
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article DNA amplification diagnostics isothermal amplification DNA 9007-49-2 DNA-Binding Proteins