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240326s2024 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.3c02958
|2 doi
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|a pubmed24n1495.xml
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|a eng
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1 |
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|a McDonough, Rowan
|e verfasserin
|4 aut
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| 245 |
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|a Kinetic Model for the Heterogeneous Biocatalytic Reactions Using Tethered Cofactors
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|c 2024
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|a Text
|b txt
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|a Date Completed 03.04.2024
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|a Date Revised 08.08.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Understanding the mechanism of interfacial enzyme kinetics is critical to the development of synthetic biological systems for the production of value-added chemicals. Here, the interfacial kinetics of the catalysis of β-nicotinamide adenine dinucleotide (NAD+)-dependent enzymes acting on NAD+ tethered to the surface of silica nanoparticles (SiNPs) has been investigated using two complementary and supporting kinetic approaches: enzyme excess and reactant (NAD+) excess. Kinetic models developed for these two approaches characterize several critical reaction steps including reversible enzyme adsorption, complexation, decomplexation, and catalysis of the surface-bound enzyme/NAD+ complex. The analysis reveals a concentrating effect resulting in a very high local concentration of enzyme and cofactor on the particle surface, in which the enzyme is saturated by surface-bound NAD, facilitating a rate enhancement of enzyme/NAD+ complexation and catalysis. This resulted in high enzyme efficiency within the tethered NAD+ system compared to that of the free enzyme/NAD+ system, which increases with decreasing enzyme concentration. The role of enzyme adsorption onto solid substrates with a tethered catalyst (such as NAD+) has potential for creating highly efficient flow biocatalytic systems
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a NAD
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|a 0U46U6E8UK
|2 NLM
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1 |
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|a Williams, Charlotte C
|e verfasserin
|4 aut
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|a Hartley, Carol J
|e verfasserin
|4 aut
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|a French, Nigel
|e verfasserin
|4 aut
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| 700 |
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|a Scott, Colin
|e verfasserin
|4 aut
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| 700 |
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|a Lewis, David A
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 40(2024), 13 vom: 02. Apr., Seite 6685-6693
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:40
|g year:2024
|g number:13
|g day:02
|g month:04
|g pages:6685-6693
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|u http://dx.doi.org/10.1021/acs.langmuir.3c02958
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