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240324s2024 xx |||||o 00| ||eng c |
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|a 10.1002/adma.202401384
|2 doi
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|a pubmed25n1233.xml
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|a eng
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| 100 |
1 |
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|a Yin, Hao
|e verfasserin
|4 aut
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| 245 |
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|a A T-Cell Inspired Sonoporation System Enhances Low-Dose X-Ray-Mediated Pyroptosis and Radioimmunotherapy Efficacy by Restoring Gasdermin-E Expression
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|c 2024
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 26.06.2024
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|a Date Revised 04.11.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2024 Wiley‐VCH GmbH.
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|a Genome editing has the potential to improve the unsatisfactory therapeutic effect of antitumor immunotherapy. However, the cell plasma membrane prevents the entry of almost all free genome-manipulation agents. Therefore, a system can be spatiotemporally controlled and can instantly open the cellular membrane to allow the entry of genome-editing agents into target cells is needed. Here, inspired by the ability of T cells to deliver cytotoxins to cancer cells by perforation, an ultrasound (US)-controlled perforation system (UPS) is established to enhance the delivery of free genome-manipulating agents. The UPS can perforate the tumor cell membrane while maintaining cell viability via a controllable lipid peroxidation reaction. In vitro, transmembrane-incapable plasmids can enter cells and perform genome editing with the assistance of UPS, achieving an efficiency of up to 90%. In vivo, the UPS is biodegradable, nonimmunogenic, and tumor-targeting, enabling the puncturing of tumor cells under US. With the application of UPS-assisted genome editing, gasdermin-E expression in 4T1 tumor-bearing mice is successfully restored, which leads to pyroptosis-mediated antitumor immunotherapy via low-dose X-ray irradiation. This study provides new insights for designing a sonoporation system for genome editing. Moreover, the results demonstrate that restoring gasdermin expression by genome editing significantly improves the efficacy of radioimmunotherapy
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|a Journal Article
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|a T‐cell bionic sonoporation
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|a genome editing
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|a low‐dose X‐ray
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| 650 |
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|a pyroptosis
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| 650 |
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4 |
|a radioimmunotherapy
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| 650 |
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|a Pore Forming Cytotoxic Proteins
|2 NLM
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| 650 |
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|a GSDME protein, human
|2 NLM
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| 650 |
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7 |
|a Gasdermins
|2 NLM
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| 700 |
1 |
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|a Hu, Xiaoqu
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Xie, Congying
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Yida
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Gao, Yanjun
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zeng, Hanqian
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhu, Wenting
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Xie, Danli
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Qinyang
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Advanced materials (Deerfield Beach, Fla.)
|d 1998
|g 36(2024), 26 vom: 09. Juni, Seite e2401384
|w (DE-627)NLM098206397
|x 1521-4095
|7 nnas
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| 773 |
1 |
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|g volume:36
|g year:2024
|g number:26
|g day:09
|g month:06
|g pages:e2401384
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| 856 |
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|u http://dx.doi.org/10.1002/adma.202401384
|3 Volltext
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