Genomic basis of the distinct biosynthesis of β-glucogallin, a biochemical marker for hydrolyzable tannin production, in three oak species

Genomic basis of the distinct biosynthesis of β-glucogallin, a biochemical marker for hydrolyzable tannin production, in three oak species

© 2024 The Authors New Phytologist © 2024 New Phytologist Foundation.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 242(2024), 6 vom: 21. Juni, Seite 2702-2718
1. Verfasser: Yang, Qinsong (VerfasserIn)
Weitere Verfasser: Li, Jinjin, Wang, Yan, Wang, Zefu, Pei, Ziqi, Street, Nathaniel R, Bhalerao, Rishikesh P, Yu, Zhaowei, Gao, Yuhao, Ni, Junbei, Jiao, Yang, Sun, Minghui, Yang, Xiong, Chen, Yixin, Liu, Puyuan, Wang, Jiaxi, Liu, Yong, Li, Guolei
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2024
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article UGT84A13 hydrolyzable tannin oak whole‐genome sequencing β‐glucogallin Biomarkers glucogallin 58511-73-2 Hydrolyzable Tannins Plant Proteins
Beschreibung
Zusammenfassung:© 2024 The Authors New Phytologist © 2024 New Phytologist Foundation.
Hydrolyzable tannins (HTs), predominant polyphenols in oaks, are widely used in grape wine aging, feed additives, and human healthcare. However, the limited availability of a high-quality reference genome of oaks greatly hampered the recognition of the mechanism of HT biosynthesis. Here, high-quality reference genomes of three Asian oak species (Quercus variabilis, Quercus aliena, and Quercus dentata) that have different HT contents were generated. Multi-omics studies were carried out to identify key genes regulating HT biosynthesis. In vitro enzyme activity assay was also conducted. Dual-luciferase and yeast one-hybrid assays were used to reveal the transcriptional regulation. Our results revealed that β-glucogallin was a biochemical marker for HT production in the cupules of the three Asian oaks. UGT84A13 was confirmed as the key enzyme for β-glucogallin biosynthesis. The differential expression of UGT84A13, rather than enzyme activity, was the main reason for different β-glucogallin and HT accumulation. Notably, sequence variations in UGT84A13 promoters led to different trans-activating activities of WRKY32/59, explaining the different expression patterns of UGT84A13 among the three species. Our findings provide three high-quality new reference genomes for oak trees and give new insights into different transcriptional regulation for understanding β-glucogallin and HT biosynthesis in closely related oak species
Beschreibung:Date Completed 23.05.2024
Date Revised 29.07.2024
published: Print-Electronic
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.19711