A nondestructive asymptomatic early disease prediction method employing ROS-induced differential volatile emissions from dry rot-infected potatoes

Copyright © 2024 Elsevier Masson SAS. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 208(2024) vom: 15. März, Seite 108532
1. Verfasser: Ray, Rittika (VerfasserIn)
Weitere Verfasser: Singh, Shiv Shakti, Yadav, Shri Ram, Sircar, Debabrata
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2024
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article Gas chromatography H(2)O(2) signaling Headspace Non-destructive disease detection ROS SPME Volatile organic compounds Reactive Oxygen Species Volatile Organic Compounds Biomarkers
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520 |a Potatoes are a staple crop with many health benefits. Postharvest storage of potatoes takes a considerable amount of time. Potato dry rot is one of the most serious postharvest storage diseases, caused primarily by the fungus Fusarium sambucinum. It is possible to minimize losses if disease is detected early, which allows it to be controlled promptly. A phytopathogen infection can alter the volatile profile of plants. Identifying unique volatile organic compounds (VOCs) as biomarkers for early disease detection is an area of considerable research interest. In this study, we compared the VOC profiles of healthy and dry rot inoculated potatoes (cv. "Kufri Pukhraj") over a time course using gas chromatography-mass spectrometry (GC-MS). There were 29 differentially emitting VOCs between healthy and dry rot inoculated potatoes. Nevertheless, only four of these compounds (linalool tetrahydride, γ-muurolene, alloaromadendrene, and α-isomethyl ionone) were exclusively found in dry rot inoculated potatoes, and hence they were considered biomarkers. Furthermore, reactive oxygen species (ROS) levels were altered in potatoes that were inoculated with dry rot, suggesting a role for ROS signaling in differential VOC emissions. In the early stages of dry rot infection, when symptoms were barely visible, these four biomarker VOCs were robustly useful in distinguishing healthy and dry rot-infected potatoes. These novel biomarkers associated with this disease are promising candidates for non-destructive detection of dry rot in stored potatoes at an early asymptomatic stage. These biomarkers can be used to develop an e-nose sensor to predict dry rot in the future 
650 4 |a Journal Article 
650 4 |a Gas chromatography 
650 4 |a H(2)O(2) signaling 
650 4 |a Headspace 
650 4 |a Non-destructive disease detection 
650 4 |a ROS 
650 4 |a SPME 
650 4 |a Volatile organic compounds 
650 7 |a Reactive Oxygen Species  |2 NLM 
650 7 |a Volatile Organic Compounds  |2 NLM 
650 7 |a Biomarkers  |2 NLM 
700 1 |a Singh, Shiv Shakti  |e verfasserin  |4 aut 
700 1 |a Yadav, Shri Ram  |e verfasserin  |4 aut 
700 1 |a Sircar, Debabrata  |e verfasserin  |4 aut 
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773 1 8 |g volume:208  |g year:2024  |g day:15  |g month:03  |g pages:108532 
856 4 0 |u http://dx.doi.org/10.1016/j.plaphy.2024.108532  |3 Volltext 
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