Divergent evolution of the alcohol-forming pathway of wax biosynthesis among bryophytes

Bibliographische Detailangaben
Veröffentlicht in:	The New phytologist. - 1979. - 242(2024), 5 vom: 09. Mai, Seite 2251-2269
1. Verfasser:	Keyl, Alisa (VerfasserIn)
Weitere Verfasser:	Herrfurth, Cornelia, Pandey, Garima, Kim, Ryeo Jin, Helwig, Lina, Haslam, Tegan M, de Vries, Sophie, de Vries, Jan, Gutsche, Nora, Zachgo, Sabine, Suh, Mi Chung, Kunst, Ljerka, Feussner, Ivo
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2024
Zugriff auf das übergeordnete Werk:	The New phytologist
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't Marchantia polymorpha Physcomitrium patens bifunctional wax ester synthase/acyl‐CoA:diacylglycerol acyltransferase cuticle evolution fatty acyl reductase Waxes Alcohols mehr... Plant Proteins Aldehyde Oxidoreductases EC 1.2.- Acyltransferases EC 2.3.-

Beschreibung
Zusammenfassung:	© 2024 The Authors. New Phytologist © 2024 New Phytologist Foundation. The plant cuticle is a hydrophobic barrier, which seals the epidermal surface of most aboveground organs. While the cuticle biosynthesis of angiosperms has been intensively studied, knowledge about its existence and composition in nonvascular plants is scarce. Here, we identified and characterized homologs of Arabidopsis thaliana fatty acyl-CoA reductase (FAR) ECERIFERUM 4 (AtCER4) and bifunctional wax ester synthase/acyl-CoA:diacylglycerol acyltransferase 1 (AtWSD1) in the liverwort Marchantia polymorpha (MpFAR2 and MpWSD1) and the moss Physcomitrium patens (PpFAR2A, PpFAR2B, and PpWSD1). Although bryophyte harbor similar compound classes as described for angiosperm cuticles, their biosynthesis may not be fully conserved between the bryophytes M. polymorpha and P. patens or between these bryophytes and angiosperms. While PpFAR2A and PpFAR2B contribute to the production of primary alcohols in P. patens, loss of MpFAR2 function does not affect the wax profile of M. polymorpha. By contrast, MpWSD1 acts as the major wax ester-producing enzyme in M. polymorpha, whereas mutations of PpWSD1 do not affect the wax ester levels of P. patens. Our results suggest that the biosynthetic enzymes involved in primary alcohol and wax ester formation in land plants have either evolved multiple times independently or undergone pronounced radiation followed by the formation of lineage-specific toolkits
Beschreibung:	Date Completed 08.05.2024 Date Revised 08.05.2024 published: Print-Electronic Citation Status MEDLINE
ISSN:	1469-8137
DOI:	10.1111/nph.19687