Metabolomics combined with physiology and transcriptomics reveal how Nicotiana tabacum leaves respond to cold stress

Copyright © 2024 Elsevier Masson SAS. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 208(2024) vom: 15. März, Seite 108464
1. Verfasser: Song, Xiliang (VerfasserIn)
Weitere Verfasser: Wang, Hui, Wang, Yujie, Zeng, Qiangcheng, Zheng, Xuebo
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2024
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article Amino acid metabolism Carbohydrate metabolism Low temperature Metabolome Nicotiana tabacum RNA-Sequencing Proline 9DLQ4CIU6V
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520 |a Low temperature-induced cold stress is a major threat to plant growth, development and distribution. Unraveling the responses of temperature-sensitive crops to cold stress and the mechanisms of cold acclimation are critical for food demand. In this study, combined physiological, transcriptomic, and metabolomic analyses were conducted on Nicotiana tabacum suffering short-term 4 °C cold stress. Our results showed that cold stress destroyed cellular membrane stability, decreased the chlorophyll (Chl) and carotenoid contents, and closed stomata, resulting in lipid peroxidation and photosynthesis restriction. Chl fluorescence measurements revealed that primary photochemistry, photoelectrochemical quenching and photosynthetic electron transport in Nicotiana tabacum leaves were seriously suppressed upon exposer to cold stress. Enzymatic and nonenzymatic antioxidants, including superoxide dismutase, catalase, peroxidase, reduced glutathione, proline, and soluble sugar, were all profoundly increased to trigger the cold acclimation defense against oxidative damage. A total of 178 metabolites and 16,204 genes were differentially expressed in cold-stressed Nicotiana tabacum leaves. MEturquoise and MEblue modules identified by WGCNA were highly correlated with physiological indices, and the corresponding hub genes were significantly enriched in pathways related to photosynthesis - antenna proteins and flavonoid biosynthesis. Untargeted metabolomic analysis identified specific metabolites, including sucrose, phenylalanine, glutamine, glutamate, and proline, that enhance plant cold acclimation. Combined transcriptomics and metabolomic analysis highlight the vital roles of carbohydrate and amino acid metabolism in enhancing the cold tolerance of Nicotiana tabacum. Our comprehensive investigation provides novel insights for efforts to alleviate low temperature-induced oxidative damage to Nicotiana tabacum plants and proposes a breeding target for cold stress-tolerant cultivars 
650 4 |a Journal Article 
650 4 |a Amino acid metabolism 
650 4 |a Carbohydrate metabolism 
650 4 |a Low temperature 
650 4 |a Metabolome 
650 4 |a Nicotiana tabacum 
650 4 |a RNA-Sequencing 
650 7 |a Proline  |2 NLM 
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700 1 |a Wang, Hui  |e verfasserin  |4 aut 
700 1 |a Wang, Yujie  |e verfasserin  |4 aut 
700 1 |a Zeng, Qiangcheng  |e verfasserin  |4 aut 
700 1 |a Zheng, Xuebo  |e verfasserin  |4 aut 
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