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|a 10.1111/jpy.13438
|2 doi
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|a eng
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|a Engstrom, Casey B
|e verfasserin
|4 aut
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|a Rosetta gen. nov. (Chlorophyta)
|b Resolving the identity of red snow algal rosettes
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|c 2024
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|a Date Completed 11.04.2024
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|a Date Revised 15.04.2024
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|a published: Print-Electronic
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|a CommentIn: J Phycol. 2024 Apr;60(2):273-274. - PMID 38597572
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|a Citation Status MEDLINE
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|a © 2024 The Authors. Journal of Phycology published by Wiley Periodicals LLC on behalf of Phycological Society of America.
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|a Thick-walled rosette-like snow algae were long thought to be a life stage of various other species of snow algae. Rosette-like cells have not been cultured, but by manually isolating cells from 38 field samples in southern British Columbia, we assigned a variety of rosette morphologies to DNA sequence. Phylogenetic analysis of Rubisco large-subunit (rbcL) gene, ribosomal internal transcribed spacer 2 (ITS2) rRNA region, and 18S rRNA gene revealed that the rosette-like cells form a new clade within the phylogroup Chloromonadinia. Based on these data, we designate a new genus, Rosetta, which comprises five novel species: R. castellata, R. floranivea, R. stellaria, R. rubriterra, and R. papavera. In a survey of 762 snow samples from British Columbia, we observed R. floranivea exclusively on snow overlying high-elevation glaciers, whereas R. castellata was observed at lower elevations, near the tree line. The other three species were rarely observed. Spherical red cells enveloped in a thin translucent sac were conspecific with Rosetta, possibly a developmental stage. These results highlight the unexplored diversity among snow algae and emphasize the utility of single-cell isolation to advance the centuries-old problem of disentangling life stages and cryptic species
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Chlainomonas
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|a Chloromonas
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|a Sanguina
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|a Chloromonadinia
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|a morphology
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|a phylogeny
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|a single‐cell PCR
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|a taxonomy
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|a ultrastructure
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|a watermelon snow
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|a RNA, Ribosomal, 18S
|2 NLM
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|a Raymond, Breanna B
|e verfasserin
|4 aut
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|a Albeitshawish, Joud
|e verfasserin
|4 aut
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|a Bogdanovic, Anastasia
|e verfasserin
|4 aut
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|a Quarmby, Lynne M
|e verfasserin
|4 aut
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|i Enthalten in
|t Journal of phycology
|d 1966
|g 60(2024), 2 vom: 22. Apr., Seite 275-298
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|g volume:60
|g year:2024
|g number:2
|g day:22
|g month:04
|g pages:275-298
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|u http://dx.doi.org/10.1111/jpy.13438
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